MIF antibody (Biotin)

Catalog No. ABIN290660

The Goat Polyclonal anti-MIF antibody has been validated for WB, ELISA and FACS. It is suitable to detect MIF in samples from Human.

Quick Overview for MIF antibody (Biotin) (ABIN290660)

Target: MIF (Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This MIF antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-MIF Antibody (Biotin)

Specificity: Recognizes recombinant and natural human Migration Inhibitory Factor (MIF). Does not cross-react with other cytokines.

Purification: Immunoaffinity purified

Immunogen: Human MIF, recombinant protein expressed in E. coli.

Type of Immunogen: Recombinant protein

Isotype: IgG

Application Details

Application Notes: Approved: ELISA, Flo, WB (0.1 - 0.2 μg/mL)

Usage: Flow Cytometry: 2.5 μg/mL labels 10e6 cells. HL-60 human acute promyelocytic leukemia cell line treated with LPS, fixed with paraformaldehyde and permeabilized with saponin.Western Blot: 0.1 μg/mL. Recombinant human MIF. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 250 μL sterile PBS

Concentration: Lot specific

Buffer: Lyophilized, PBS,  pH 7.2, BSA

Storage: -20 °C

Storage Comment: Lyophilized powder may be stored at -20°C for short-term only. Reconstituted product is stable for 1 year at -20°C.

Target Details for MIF

Target: MIF (Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF))

Alternative Name: MIF

Background: Name/Gene ID: MIF

Synonyms: MIF, GLIF, L-dopachrome tautomerase, L-dopachrome isomerase, MMIF, Phenylpyruvate tautomerase

Gene ID: 4282

UniProt: P14174

Pathways: Regulation of Systemic Arterial Blood Pressure by Hormones, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling