TGFB1 antibody (Biotin)

Catalog No. ABIN290963

The Chicken Polyclonal anti-TGFB1 antibody has been validated for WB and ELISA. It is suitable to detect TGFB1 in samples from Human and Mouse.

Quick Overview for TGFB1 antibody (Biotin) (ABIN290963)

Target: TGFB1 (Transforming Growth Factor, beta 1 (TGFB1))

Reactivity: Human, Mouse

Host: Chicken

Clonality: Polyclonal

Conjugate: This TGFB1 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Product Details anti-TGFB1 Antibody (Biotin)

Specificity: Recognizes human TGFb1. When used in combination with the monoclonal capture antibody LS-C10854 in a sandwich ELISA, ~5 % cross-reactivity with TGF-b1.2 and 1 % cross-reactivity with rhTGF-b2, rcTGF-b3 and raTG-b5 was observed.

Purification: Affinity purified

Immunogen: Recombinant human TGF-b1 expressed in CHO cells.

Type of Immunogen: Recombinant protein

Isotype: IgY

Application Details

Application Notes: Approved: ELISA, WB (0.1 - 0.2 μg/mL)

Usage: Suitable for use in ELISA and Western Blot. Plates are coated with 100 μL/well of the capture antibody in combination with 100 μL/well of the detection antibody at 300 ng/mL. An ELISA for sample volumes of 100 μL can be obtained. Titrate each preparation of the recombinant protein for standard preparation to arrive at the most suitable dose range. For this antibody pair, a two-fold dilution series starting at 2 ng/mL is suggested. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Sterile PBS. Resuspend to 250 μL

Concentration: Lot specific

Buffer: Supplied as a lyopholized powder from PBS,  pH 7.4, 50 μg BSA.

Handling Advice: Avoid frequent freeze thaws. Store undiluted.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for TGFB1

Target: TGFB1 (Transforming Growth Factor, beta 1 (TGFB1))

Alternative Name: TGFB1 / TGF Beta 1

Background: Name/Gene ID: TGFB1
Family: TGF beta

Synonyms: TGFB1, CED, DPD1, Diaphyseal dysplasia 1, LAP, Latency-associated peptide, TGF Beta, TGFbeta, TGF-beta 1 protein, TGF-beta-1, TGFB

Gene ID: 7040

UniProt: P01137

Pathways: EGFR Signaling Pathway, Dopaminergic Neurogenesis, Cellular Response to Molecule of Bacterial Origin, Glycosaminoglycan Metabolic Process, Regulation of Leukocyte Mediated Immunity, Regulation of Muscle Cell Differentiation, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization, Production of Molecular Mediator of Immune Response, Ribonucleoside Biosynthetic Process, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy, Cancer Immune Checkpoints