Keratin 10/13 antibody

Catalog No. ABIN291339

The Mouse Monoclonal anti-Keratin 10/13 antibody (Clone DE-K13) (ABIN291339) specifically detects Keratin 10/13 in WB, IHC, IHC (p) and IHC (fro). The antibody is reactive with Human samples.

Quick Overview for Keratin 10/13 antibody (ABIN291339)

Target: Keratin 10/13

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: DE-K13

Product Details

Specificity: Reacts with Cytokeratin 10 (56.5 kD) and Cytokeratin 13 (53 kD). Cytokeratins are a member of intermediate filaments subfamily represented in epithelial tissues. DE-K13 recognizes only Cytokeratin 13 on formalin-fixed, paraffin-embedded tissue sections.

Purification: Protein A purified

Immunogen: Cytoskeletal preparation extracted from human ectocervical epithelium.

Type of Immunogen: Protein fraction

Isotype: IgG2a

Application Details

Application Notes: Approved: IHC, IHC-Fr, IHC-P, WB (1:100, 1 - 3 μg/mL)

Usage: Western Blotting: Recommended dilution - 1-3 μg/mL. Immunohistochemistry (paraffin sections): Staining technique: Standard ABC technique (DAB+). Recommended dilution - 10 μg/mL (1:100). Positive tissue - tonsil. Pretreatment: 0.1 % pepsin in 0.1 M HCl for

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 15 mM sodium azide, approx,  pH 7.4.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details

Target: Keratin 10/13

Alternative Name: Cytokeratin 10+13

Background: This antibody detects: Cytokeratin 10+13