RIPK3 antibody (AA 480-530)

Catalog No. ABIN291732

The Rabbit Polyclonal anti-RIPK3 antibody (ABIN291732) specifically detects RIPK3 in WB, IHC and IHC (p). The antibody is reactive with Human samples.

Quick Overview for RIPK3 antibody (AA 480-530) (ABIN291732)

Target: RIPK3 (Receptor-Interacting serine-threonine Kinase 3 (RIPK3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RIPK3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-RIPK3 Antibody

Binding Specificity: AA 480-530

Specificity: A portion of amino acids 480-530 of human RIP3.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla (100%) Gibbon (93%).

Purification: Immunoaffinity purified

Immunogen: A synthetic peptide (QEGPKDPEAWSRPQ) corresponding to amino acids 497-510 (isoform CRA_a, 518 amino acids) and 298-311 (isoform CRA_b, 319 amino acids) of human RIP3 was used as immunogen for this antibody. Percent identity by BLAST analysis: Human, Gorilla (100%), Gibbon (93%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (10 μg/mL), WB (1 - 3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.05 % BSA, 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for RIPK3

Target: RIPK3 (Receptor-Interacting serine-threonine Kinase 3 (RIPK3))

Alternative Name: RIPK3 / RIP3

Background: Name/Gene ID: RIPK3
Subfamily: RIP
Family: Protein Kinase

Synonyms: RIPK3, Receptor interacting protein 3, RIP-like protein kinase 3, RIP3, RIP-3, Receptor-interacting protein 3

Gene ID: 11035

UniProt: Q9Y572

Pathways: Activation of Innate immune Response, Toll-Like Receptors Cascades