Keratin 7/17 antibody

Catalog No. ABIN291845

The Mouse Monoclonal anti-Keratin 7/17 antibody is suitable to detect Keratin 7/17 in samples from Human and Cow. It has been validated for IHC, WB, ICC, IHC (p) and IP.

Quick Overview for Keratin 7/17 antibody (ABIN291845)

Target: Keratin 7/17 (KRT7/17)

Reactivity: Human, Cow

Host: Mouse

Clonality: Monoclonal

Conjugate: This Keratin 7/17 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Clone: C-46

Product Details anti-Keratin 7/17 Antibody

Specificity: Reacts with Cytokeratin peptides 7 and 17 (54 and 46 kD). Cytokeratins are a member of intermediate filaments subfamily represented in epithelial tissues.

Purification: Protein A purified

Immunogen: Cytoskeleton preparation of HeLa human cervix carcinoma cell line.

Isotype: IgG1

Application Details

Application Notes: Approved: ICC, IHC, IHC-P, IP (2 - 3 μg/mL), WB (2 - 3 μg/mL)

Usage: Western Blotting: Recommended dilution - 1 μg/mL. Positive control - HeLa human cell line. Sample preparation:Lysis 1 % lauryl maltoside for 1 hour, 5x10 sec. sonication. 5 min. in hot Laemmli buffer (reducing conditions.). Immunohistochemistry (paraffin s

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 15 mM sodium azide, approx,  pH 7.4.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for Keratin 7/17

Target: Keratin 7/17 (KRT7/17)

Alternative Name: Cytokeratin 7+17

Background: This antibody detects: Cytokeratin 7+17