Cytokeratin 19 antibody

Catalog No. ABIN292298

The Mouse Monoclonal anti-Cytokeratin 19 antibody is suitable to detect Cytokeratin 19 in samples from Human. It has been validated for WB, IHC, ELISA, IHC (p), ICC and IP.

Quick Overview for Cytokeratin 19 antibody (ABIN292298)

Target: Cytokeratin 19 (KRT19) (Keratin 19 (KRT19))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cytokeratin 19 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Clone: A53-B-A2

Product Details anti-Cytokeratin 19 Antibody

Specificity: Human mammary carcinoma cell line MCF-7

Purification: Affinity purified

Immunogen: Cells.

Isotype: IgG2a

Application Details

Application Notes: Approved: ELISA, ICC, IHC, IHC-P (10 μg/mL), IP, WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Phosphate-buffered solution,  pH 7.2, 0.09 % sodium azide at 0.5 mg/mL.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: 4°C. Store undiluted.

Target Details for Cytokeratin 19

Target: Cytokeratin 19 (KRT19) (Keratin 19 (KRT19))

Alternative Name: KRT19 / CK19 / Cytokeratin 19

Background: Name/Gene ID: KRT19

Synonyms: KRT19, CK19, Cytokeratin 19, Cytokeratin-19, Keratin 19, Keratin-19, CK-19, K19, K1CS, Keratin, type I, 40-kd

Gene ID: 3880

UniProt: P08727