GLI3 antibody
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- Target See all GLI3 Antibodies
- GLI3 (GLI Family Zinc Finger 3 (GLI3))
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Reactivity
- Human, Mouse
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- This GLI3 antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunocytochemistry (ICC)
- Specificity
- Recognizes human and mouse GLI-3 in the applications listed. In direct ELISAs and Western Blots, the antibody shows less than 1 % cross-reactivity with rhGLI-1 and rhGLI-2.
- Purification
- Affinity purified
- Immunogen
- E. coli-derived rhGLI-3.
- Isotype
- IgG
- Top Product
- Discover our top product GLI3 Primary Antibody
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- Application Notes
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Approved: ELISA (0.5 - 1 μg/mL), ICC (10 μg/mL), WB (0.1 - 0.2 μg/mL)
Usage: Suitable for use in ELISA, Immunocytochemistry, and Western Blot. Western Blot: 0.1-0.2 μg/mL. Used in conjunction with the appropriate secondary reagents, detects human and mouse GLI-3. The detection limit for rhGLI-3 and rmGLI-3 is approximately 5 ng/lane under non- reducing and reducing conditions. Immunocytochemistry: 10 μg/mL detects human GLI-3 in HeLa cells. Cells were fixed with PBS containing 4 % paraformaldehyde and blocked with PBS containing 10 % normal donkey serum, 0.1 % Triton X-100, and 1 % BSA. After blocking, cells were incubated with diluted primary antibody followed by NorthernLights 557-coupled anti-goat IgG at room temperature in the dark. Between each step, cells were washed with PBS containing BSA. Direct ELISA: 0.5-1.0 μg/mL. Used in conjunction the appropriate secondary reagents, detects human and mouse GLI-3. The detection limit for rhGLI-3 and rmGLI-3 is approximately 1 ng/well. - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- deionized water
- Concentration
- Lot specific
- Buffer
- Lyophilized.
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- Target
- GLI3 (GLI Family Zinc Finger 3 (GLI3))
- Alternative Name
- GLI3 (GLI3 Products)
- Synonyms
- ACLS antibody, GCPS antibody, GLI3-190 antibody, GLI3FL antibody, PAP-A antibody, PAPA antibody, PAPA1 antibody, PAPB antibody, PHS antibody, PPDIV antibody, xgli3 antibody, AI854843 antibody, AU023367 antibody, Bph antibody, Pdn antibody, Xt antibody, add antibody, im:7142603 antibody, GLI3 antibody, GLI family zinc finger 3 antibody, GLI family zinc finger 3 L homeolog antibody, GLI-Kruppel family member GLI3 antibody, GLI3 antibody, Gli3 antibody, gli3.L antibody, gli3 antibody
- Background
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Name/Gene ID: GLI3
Synonyms: GLI3, ACLS, GCPS, GLI-Kruppel family member GLI3, GLI3 full length protein, GLI3-190, GLI family zinc finger 3, Gli-3, GLI3FL, PAP-A, PAPA1, PHS, PPDIV, Transcriptional activator GLI3, PAPA, PAPB, GLI3 form of 190 kDa, Oncogene GLI3, Zinc finger protein GLI3 - Gene ID
- 2737
- UniProt
- P10071
- Pathways
- Hedgehog Signaling
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