Tyrosine Hydroxylase antibody

Catalog No. ABIN293223

The Sheep Polyclonal anti-Tyrosine Hydroxylase antibody (ABIN293223) specifically detects Tyrosine Hydroxylase in WB and IHC. The antibody is reactive with Rat samples.

Quick Overview for Tyrosine Hydroxylase antibody (ABIN293223)

Target: Tyrosine Hydroxylase (TH)

Reactivity: Rat

Host: Sheep

Clonality: Polyclonal

Conjugate: This Tyrosine Hydroxylase antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-Tyrosine Hydroxylase Antibody

Specificity: Recognizes tyrosine hydroxylase. Cross-reacts with all mammalian forms and some non-mammalian forms.

Purification: Affinity purified

Immunogen: Native tyrosine hydroxylase from rat pheochromocytoma.

Type of Immunogen: Purified protein

Isotype: IgG

Application Details

Application Notes: Approved: IHC (0.02 - 0.1 μg/mL), WB (0.05 - 0.1 μg/mL)

Usage: Suitable for use in Western Blot and Immunohistochemistry (peroxidase). Western Blot: 0.05-0.1 μg/mL readily detected 1-10 ng of TH/lane using 125I-protein A. Immunohistochemistry: 0.02-0.1 μg/mL produced specific labeling of noradrenergic axons in primate cerebral cortex. Using horseradish peroxidase (HRP). Note: It is important to use a secondary antibody that has significant reactivity to sheep IgG1 since the majority of the sheep immunoglobulin is IgG1.

Comment:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Dulbeccos PBS, 10 % glycerol, 10 mM HEPES, 20 mM Tris-HCl, 0.1 % PVP-40, 0.05 % Tween-20, 0.1 % sodium azide, 1 mg/mL BSA.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for Tyrosine Hydroxylase

Target: Tyrosine Hydroxylase (TH)

Alternative Name: TH / Tyrosine Hydroxylase

Background: Name/Gene ID: TH
Family: Monooxygenase

Synonyms: TH, DYT14, Dystonia 14, TYH, Tyrosine 3-hydroxylase, Tyrosine hydroxylase, DYT5b, Tyrosine 3-monooxygenase

Gene ID: 7054

UniProt: P07101

Pathways: Dopaminergic Neurogenesis, Response to Water Deprivation, Sensory Perception of Sound, Carbohydrate Homeostasis, Feeding Behaviour