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Tyrosine Hydroxylase antibody

This anti-Tyrosine Hydroxylase antibody is a Sheep Polyclonal antibody detecting Tyrosine Hydroxylase in WB and IHC. Suitable for Rat.
Catalog No. ABIN293223

Quick Overview for Tyrosine Hydroxylase antibody (ABIN293223)

Target

See all Tyrosine Hydroxylase (TH) Antibodies
Tyrosine Hydroxylase (TH)

Reactivity

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Rat

Host

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Sheep

Clonality

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Polyclonal

Conjugate

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This Tyrosine Hydroxylase antibody is un-conjugated

Application

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Western Blotting (WB), Immunohistochemistry (IHC)
  • Specificity

    Recognizes tyrosine hydroxylase. Cross-reacts with all mammalian forms and some non-mammalian forms.

    Purification

    Affinity purified

    Immunogen

    Native tyrosine hydroxylase from rat pheochromocytoma.

    Type of Immunogen: Purified protein

    Isotype

    IgG
  • Application Notes

    Approved: IHC (0.02 - 0.1 μg/mL), WB (0.05 - 0.1 μg/mL)

    Usage: Suitable for use in Western Blot and Immunohistochemistry (peroxidase). Western Blot: 0.05-0.1 μg/mL readily detected 1-10 ng of TH/lane using 125I-protein A. Immunohistochemistry: 0.02-0.1 μg/mL produced specific labeling of noradrenergic axons in primate cerebral cortex. Using horseradish peroxidase (HRP). Note: It is important to use a secondary antibody that has significant reactivity to sheep IgG1 since the majority of the sheep immunoglobulin is IgG1.

    Comment

    Target Species of Antibody: Rat

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    Lot specific

    Buffer

    Dulbeccos PBS, 10 % glycerol, 10 mM HEPES, 20 mM Tris-HCl, 0.1 % PVP-40, 0.05 % Tween-20, 0.1 % sodium azide, 1 mg/mL BSA.

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handling Advice

    Avoid repeat freeze-thaw cycles.

    Storage

    4 °C,-20 °C

    Storage Comment

    Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
  • Target

    Tyrosine Hydroxylase (TH)

    Alternative Name

    TH / Tyrosine Hydroxylase

    Background

    Name/Gene ID: TH
    Family: Monooxygenase

    Synonyms: TH, DYT14, Dystonia 14, TYH, Tyrosine 3-hydroxylase, Tyrosine hydroxylase, DYT5b, Tyrosine 3-monooxygenase

    Gene ID

    7054

    UniProt

    P07101

    Pathways

    Dopaminergic Neurogenesis, Response to Water Deprivation, Sensory Perception of Sound, Carbohydrate Homeostasis, Feeding Behaviour
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