Tyrosine Hydroxylase antibody
Quick Overview for Tyrosine Hydroxylase antibody (ABIN293223)
Target
See all Tyrosine Hydroxylase (TH) AntibodiesReactivity
Host
Clonality
Conjugate
Application
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Specificity
- Recognizes tyrosine hydroxylase. Cross-reacts with all mammalian forms and some non-mammalian forms.
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Purification
- Affinity purified
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Immunogen
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Native tyrosine hydroxylase from rat pheochromocytoma.
Type of Immunogen: Purified protein -
Isotype
- IgG
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Application Notes
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Approved: IHC (0.02 - 0.1 μg/mL), WB (0.05 - 0.1 μg/mL)
Usage: Suitable for use in Western Blot and Immunohistochemistry (peroxidase). Western Blot: 0.05-0.1 μg/mL readily detected 1-10 ng of TH/lane using 125I-protein A. Immunohistochemistry: 0.02-0.1 μg/mL produced specific labeling of noradrenergic axons in primate cerebral cortex. Using horseradish peroxidase (HRP). Note: It is important to use a secondary antibody that has significant reactivity to sheep IgG1 since the majority of the sheep immunoglobulin is IgG1. -
Comment
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Target Species of Antibody: Rat
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- Lot specific
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Buffer
- Dulbeccos PBS, 10 % glycerol, 10 mM HEPES, 20 mM Tris-HCl, 0.1 % PVP-40, 0.05 % Tween-20, 0.1 % sodium azide, 1 mg/mL BSA.
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Preservative
- Sodium azide
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Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Handling Advice
- Avoid repeat freeze-thaw cycles.
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Storage
- 4 °C,-20 °C
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Storage Comment
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- Tyrosine Hydroxylase (TH)
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Alternative Name
- TH / Tyrosine Hydroxylase
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Background
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Name/Gene ID: TH
Family: Monooxygenase
Synonyms: TH, DYT14, Dystonia 14, TYH, Tyrosine 3-hydroxylase, Tyrosine hydroxylase, DYT5b, Tyrosine 3-monooxygenase -
Gene ID
- 7054
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UniProt
- P07101
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Pathways
- Dopaminergic Neurogenesis, Response to Water Deprivation, Sensory Perception of Sound, Carbohydrate Homeostasis, Feeding Behaviour
Target
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