LAT antibody (Cytoplasmic Domain)

Catalog No. ABIN293627

The Mouse Monoclonal anti-LAT antibody has been validated for WB, IHC, IP and IHC (p). It is suitable to detect LAT in samples from Human.

Quick Overview for LAT antibody (Cytoplasmic Domain) (ABIN293627)

Target: LAT (Linker For Activation of T Cells (LAT))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This LAT antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: LAT1111

Product Details anti-LAT Antibody

Binding Specificity: Cytoplasmic Domain

Brand: IHC-plus™

Specificity: Partial recombinant protein (cytoplasmic domain of human LAT)

Purification: Affinity purified

Immunogen: Recombinant human LAT.

Type of Immunogen: Recombinant protein

Isotype: IgG1

Application Details

Application Notes: Approved: IHC, IHC-P (10 μg/mL), IP, WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Phosphate-buffered solution,  pH 7.2, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 4°C.

Target Details for LAT

Target: LAT (Linker For Activation of T Cells (LAT))

Alternative Name: LAT1 / LAT

Background: Name/Gene ID: LAT

Synonyms: LAT, p36-38, Pp36, LAT1

Gene ID: 27040

UniProt: O43561

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway