IKZF1 antibody (C-Term) (PE)

Catalog No. ABIN2967182

This Mouse Monoclonal antibody specifically detects IKZF1 in ICFC. It exhibits reactivity toward Human and Mouse.

Quick Overview for IKZF1 antibody (C-Term) (PE) (ABIN2967182)

Target: IKZF1 (IKAROS Family Zinc Finger 1 (Ikaros) (IKZF1))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This IKZF1 antibody is conjugated to PE

Application: Intracellular Flow Cytometry (ICFC)

Clone: 4E9

Product Details anti-IKZF1 Antibody (PE)

Binding Specificity: C-Term

Purpose: Anti-Ikaros PE

Specificity: The mouse monoclonal antibody 4E9 recognizes Ikaros, a transcription factor (intracellular antigen) expressed broadly in hematopoietic progenitors and serving as a key regulator of lymphopoiesis.

Cross-Reactivity (Details): Human, Mouse

Purification: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen: Recombinant human Ikaros (C-terminal part)

Isotype: IgG1

Application Details

Application Notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests. Intracellular staining.

Restrictions: For Research Use only

Handling

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Target Details for IKZF1

Target: IKZF1 (IKAROS Family Zinc Finger 1 (Ikaros) (IKZF1))

Alternative Name: Ikaros

Background: IKAROS family zinc finger 1,Ikaros, also known as IKZF1 (Ikaros family zinc finger protein 1) is a hematopoietic-specific transcription factor involved in the regulation of lymphocyte development, together with other members of this family, such as Aiolos and Helios. Ikaros forms homo- and heterodimers with these proteins and functions predominantly in early hematopoietic development. Expression of Ikaros, Aiolos and Helios is restricted to cells of the hematopoietic system, whereas other family members, Eos and Pegassus, are more widely expressed. Disruption of Ikaros leads to T and B cell leukemias.,IKZF1, IK1, LYF1, LyF-1, IKAROS, PPP1R92, PRO0758, ZNFN1A1, Hs.54452, IKAROS family zinc finger 1, Lymphoid transcription factor LyF-1

Gene ID: 10320

UniProt: Q13422

Pathways: Production of Molecular Mediator of Immune Response