CD59 antibody

Catalog No. ABIN298471

This Mouse Monoclonal antibody specifically detects CD59 in WB, IHC, FACS, ELISA, IP, IHC (p) and IHC (fro). It exhibits reactivity toward Human.

Quick Overview for CD59 antibody (ABIN298471)

Target: CD59

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD59 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), ELISA, Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: MEM-43

Product Details anti-CD59 Antibody

Purification: Purified IgG

Isotype: IgG2a

Application Details

Application Notes: Approved: ELISA, Flo, IHC, IHC-Fr, IHC-P (5 μg/mL), IP, WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Flow Cytometry: Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Immunohistochemistry: This product requires antigen retrieval using heat treatment methods prior to staining of paraffin sections. Sodium citrate buffer  pH 6.0 is recommended for this purpose. Histology Positive Control Tissue: Tonsil.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details for CD59

Target: CD59

Alternative Name: CD59

Background: Name/Gene ID: CD59

Synonyms: CD59, 16.3A5, 1F5, 1F5 antigen, CD59 glycoprotein, EL32, EJ16, G344, HRF-20, MAC-IP, MACIF, Ly-6-like protein, MEM43, MIRL, MEM43 antigen, MIN2, MIN3, MSK21, p18-20, Protectin, MAC-inhibitory protein, MIC11, T cell-activating protein, CD59 antigen, EJ30, HRF20, Human leukocyte antigen MIC11, Lymphocytic antigen CD59/MEM43, MIN1

Gene ID: 966

UniProt: P13987

Pathways: Complement System