TLR4 antibody (AA 100-200)

Catalog No. ABIN298531

This Mouse Monoclonal antibody specifically detects TLR4 in WB, FACS, IHC and IHC (p). It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for TLR4 antibody (AA 100-200) (ABIN298531)

Target: TLR4 (Toll-Like Receptor 4 (TLR4))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This TLR4 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 76B357-1

Product Details anti-TLR4 Antibody

Binding Specificity: AA 100-200

Brand: IHC-plus™

Specificity: The amino acid sequence used has 100 % identity in human, chimp, baboon, Chinese hamster, and rat, 93 % identity in bovine and pig, 85 % identity in sheep and 78 % identity in cat, horse and mouse.

Purification: Protein G purified

Immunogen: Synthetic peptide from human TLR4.

Type of Immunogen: Synthetic peptide

Isotype: IgG2b kappa

Application Details

Application Notes: Approved: Flo, IHC, IHC-P (5 μg/mL), WB (1 - 3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for TLR4

Target: TLR4 (Toll-Like Receptor 4 (TLR4))

Alternative Name: TLR4

Background: Name/Gene ID: TLR4
Family: Toll-like Receptor

Synonyms: TLR4, CD284, CD284 antigen, Homolog of Drosophila toll, TLR-4, TOLL, ARMD10, Toll-like receptor 4, HToll

Gene ID: 7099

UniProt: O00206

Pathways: TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Toll-Like Receptors Cascades, Inflammasome, S100 Proteins