MECP2 antibody (AA 11-25)

Catalog No. ABIN300092

The Rabbit Polyclonal anti-MECP2 antibody is suitable to detect MECP2 in samples from Human. It has been validated for WB, IHC and IHC (p).

Quick Overview for MECP2 antibody (AA 11-25) (ABIN300092)

Target: MECP2 (Methyl CpG Binding Protein 2 (MECP2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MECP2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-MECP2 Antibody

Binding Specificity: AA 11-25

Specificity: A mixture of synthetic peptides corresponding to amino acids 11-25 and 181-195 of human MeCP2.

Purification: Protein G purified

Immunogen: Synthetic peptide from human MECP2.

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (2 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.2 % gelatin, 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for MECP2

Target: MECP2 (Methyl CpG Binding Protein 2 (MECP2))

Alternative Name: MECP2

Background: Name/Gene ID: MECP2

Synonyms: MECP2, AUTSX3, Methyl-CpG-binding protein 2, MRX16, MRX79, MRXSL, RTT, RTS, MeCp-2 protein, RS, MRXS13, PPMX

Gene ID: 4204

UniProt: P51608

Pathways: Inositol Metabolic Process, Chromatin Binding, Synaptic Membrane