FZD2 antibody (Extracellular Domain) (Biotin)

Catalog No. ABIN301677

This anti-FZD2 antibody is a Goat Polyclonal antibody detecting FZD2 in WB. Suitable for Mouse.

Quick Overview for FZD2 antibody (Extracellular Domain) (Biotin) (ABIN301677)

Target: FZD2 (Frizzled Family Receptor 2 (FZD2))

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This FZD2 antibody is conjugated to Biotin

Application: Western Blotting (WB)

Product Details anti-FZD2 Antibody (Biotin)

Binding Specificity: Extracellular Domain

Specificity: Recognizes mouse Frizzled 2, extracellular domain specific.

Purification: Immunoaffinity purified

Immunogen: NS0-derived rmFrizzled-2 extracellular Cysteine-Rich Domain (CRD).

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.1 - 0.2 μg/mL)

Usage: Suitable for use in Western Blot. Western Blot: Use at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect mouse Frizzled-2. The detection limit for rmFrizzled 2 is ~10 ng/lane and 2 ng/lane under non-reducing. and reducing conditions. In this format, this antibody shows ~25 % cross-reactivity with rmFrizzled 1 and less than 5 % cross-reactivity with rmFrizzled 3, rmFrizzled 4, rmFrizzled 5, rmFrizzled 6, rmFrizzled 7 and rmFrizzled 8. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: deionized water

Concentration: Lot specific

Buffer: Lyophilized.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Target Details for FZD2

Target: FZD2 (Frizzled Family Receptor 2 (FZD2))

Alternative Name: FSD2 / Frizzled 2

Background: Name/Gene ID: FZD2
Subfamily: Frizzled
Family: GPCR

Synonyms: FZD2, Dfrizzled2, Fz2, Frizzled family receptor 2, Frizzled-2, HFz2, Frizzled homolog 2, Fz-2, FzE2, Mfz10, Rfz2, Frizzled 2

Gene ID: 2535

UniProt: Q14332

Pathways: WNT Signaling