STAT1 antibody (Signal Transducer and Activator of Transcription 1, 91kDa)

Details for Product anti-STAT1 Antibody No. ABIN3020615
Target
Reactivity
Human, Mouse, Rat
293
160
155
24
24
23
20
18
13
10
8
6
5
5
2
1
1
1
1
1
Host
Rabbit
237
63
2
2
1
Clonality
Polyclonal
Conjugate
This STAT1 antibody is un-conjugated
15
11
11
10
9
9
9
8
8
5
5
4
4
4
4
4
3
2
2
2
Application
Immunohistochemistry (IHC), Western Blotting (WB)
207
96
87
60
55
48
23
22
11
8
4
4
4
1
1
Options
Immunogen Recombinant protein of human STAT1
Isotype IgG
Purification Affinity purification
Target
Alternative Name STAT1 (STAT1 Antibody Abstract)
Background The Stat1 transcription factor is activated in response to a large number of ligands and is essential for responsiveness to IFN-alpha and IFN-gamma. Phosphorylation of Stat1 at Tyr701 induces Stat1 dimerization, nuclear translocation, and DNA binding. Stat1 protein exists as a pair of isoforms, Stat1alpha (91 kDa) and the splice variant Stat1beta (84 kDa). In most cells, both isoforms are activated by IFN-alpha, but only Stat1alpha is activated by IFN-gamma. The inappropriate activation of Stat1 occurs in many tumors. In addition to tyrosine phosphorylation, Stat1 is also phosphorylated at Ser727 through a p38 mitogen-activated protein kinase (MAPK)-dependent pathway in response to IFN-alpha and other cellular stresses. Serine phosphorylation may be required for the maximal induction of Stat1-mediated gene activation.
Synonyms: STAT1, DKFZp686B04100, ISGF-3, STAT91
Gene ID 6772
UniProt P42224
Pathways JAK-STAT Signaling, RTK Signaling, Interferon-gamma Pathway, Response to Growth Hormone Stimulus, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity, Hepatitis C, CXCR4-mediated Signaling Events
Application Notes Recommended dilutions: WB: 1:500-1:2000, IHC: 1:50-1:200
Restrictions For Research Use only
Format Liquid
Buffer PBS with 0.02 % sodium azide, 50 % glycerol, pH 7.3
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze / thaw cycles
Storage -20 °C/-80 °C
Images
Image no. 1 for anti-Signal Transducer and Activator of Transcription 1, 91kDa (STAT1) antibody (ABIN3020615) anti-Signal Transducer and Activator of Transcription 1, 91kDa (STAT1) antibody (Image 1)
Image no. 2 for anti-Signal Transducer and Activator of Transcription 1, 91kDa (STAT1) antibody (ABIN3020615) Western blot analysis of various cell lines, using STAT1 antibody.
Product cited in: Kong, Shen, Liu, Zuo, Ji, Lu, Nakamura, Lazarus, Stratakis, Breyer, Yu: "PKA regulatory IIα subunit is essential for PGD2-mediated resolution of inflammation." in: The Journal of experimental medicine, Vol. 213, Issue 10, pp. 2209-26, 2017 (PubMed). Method employed by authors: Western Blotting (WB)

Kong, Li, Shen, Liu, Zuo, Tao, Ji, Lu, Lazarus, Breyer, Yu: "Niacin Promotes Cardiac Healing after Myocardial Infarction through Activation of the Myeloid Prostaglandin D2 Receptor Subtype 1." in: The Journal of pharmacology and experimental therapeutics, Vol. 360, Issue 3, pp. 435-444, 2017 (PubMed). Method employed by authors: Western Blotting (WB)

Guo, Hu, Chen, Li, Ye, Cheng, Zhang, He: "iTRAQ-based comparative proteomic analysis of Vero cells infected with virulent and CV777 vaccine strain-like strains of porcine epidemic diarrhea virus." in: Journal of proteomics, Vol. 130, pp. 65-75, 2016 (PubMed). Method employed by authors: Western Blotting (WB)