ERK1 antibody (AA 1-100)

Catalog No. ABIN3020725

The Rabbit Polyclonal anti-ERK1 antibody has been validated for WB, IHC and IF. It is suitable to detect ERK1 in samples from Human. There are 8+ publications available.

Quick Overview for ERK1 antibody (AA 1-100) (ABIN3020725)

Target: ERK1 (MAPK3) (Mitogen-Activated Protein Kinase 3 (MAPK3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ERK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-ERK1 Antibody

Binding Specificity: AA 1-100

Sequence: MAAAAAQGGG GGEPRRTEGV GPGVPGEVEM VKGQPFDVGP RYTQLQYIGE GAYGMVSSAY DHVRKTRVAI KKISPFEHQT YCQRTLREIQ ILLRFRHENV

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Polyclonal Antibodies

Purification: Affinity purification

Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human ERK1 (NP_002737.2).

Isotype: IgG

Application Details

Application Notes: WB,1:500 - 1:2000,IHC,1:50 - 1:200,IF,1:50 - 1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide,50 % glycerol, pH 7.3.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze / thaw cycles

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

References for anti-ERK1 antibody (ABIN3020725)

He, Zhao, Anees, Li, Ashraf, Chen, Song, Chen, Cao, Ye: "p21-Activated Kinase 4 Signaling Promotes Japanese Encephalitis Virus-Mediated Inflammation in Astrocytes." in: Frontiers in cellular and infection microbiology, Vol. 7, pp. 271, (2018) (PubMed).

Zhu, Ye, Ashraf, Li, Chen, Song, Cao: "Transcriptional regulation of miR-15b by c-Rel and CREB in Japanese encephalitis virus infection." in: Scientific reports, Vol. 6, pp. 22581, (2017) (PubMed).

Li, Fan, Gao, Fan, Liu: "Effect of Fructus Ligustri Lucidi on osteoblastic like cell-line MC3T3-E1." in: Journal of ethnopharmacology, Vol. 170, pp. 88-95, (2016) (PubMed).

Wu, Qin, Guo, Li, Chen: "AKT/ERK activation is associated with gastric cancer cell resistance to paclitaxel." in: International journal of clinical and experimental pathology, Vol. 7, Issue 4, pp. 1449-58, (2015) (PubMed).

Fan, Zhang, Hu, Li, Zhang: "Activation of AKT/ERK confers non-small cell lung cancer cells resistance to vinorelbine." in: International journal of clinical and experimental pathology, Vol. 7, Issue 1, pp. 134-43, (2014) (PubMed).

Feng, Yu, Liu, Zhang, Cheng, Li, Tan, Luo, Jiang: "KAT5 silencing induces apoptosis of GBC-SD cells through p38MAPK-mediated upregulation of cleaved Casp9." in: International journal of clinical and experimental pathology, Vol. 7, Issue 1, pp. 80-91, (2014) (PubMed).

Li, Wang, Pan, Wang, Fan, Sun: "RNAi screening with shRNAs against histone methylation-related genes reveals determinants of sorafenib sensitivity in hepatocellular carcinoma cells." in: International journal of clinical and experimental pathology, Vol. 7, Issue 3, pp. 1085-92, (2014) (PubMed).

Xu, Hang, Hu, Gao: "RAF1-MEK1-ERK/AKT axis may confer NSCLC cell lines resistance to erlotinib." in: International journal of clinical and experimental pathology, Vol. 6, Issue 8, pp. 1493-504, (2013) (PubMed).

Target Details for ERK1

Target: ERK1 (MAPK3) (Mitogen-Activated Protein Kinase 3 (MAPK3))

Alternative Name: MAPK3

Background: The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described.,ERK-1,ERK1,ERT2,HS44KDAP,HUMKER1A,P44ERK1,P44MAPK,PRKM3,p44-ERK1,p44-MAPK,MAPK3,Epigenetics & Nuclear Signaling,Translation Control,Regulation of eIF4 and p70 S6 Kinase,Signal Transduction,G protein signaling,G2/M DNA Damage Checkpoint,Kinase,Serine/threonine kinases,mTOR Signaling Pathway,ErbB-HER Signaling Pathway,MAPK-Erk Signaling Pathway,Cell Biology & Developmental Biology,Apoptosis,Mitochondrial Control of Apoptosis,Inhibition of Apoptosis,Cell Cycle,Centromere,Microtubules,TGF-b-Smad Signaling Pathway,ESC Pluripotency and Differentiation,Endocrine & Metabolism,Insulin Receptor Signaling Pathway,Warburg Effect,Immunology & Inflammation,B Cell Receptor Signaling Pathway,T Cell Receptor Signaling Pathway,IL-6 Receptor Signaling Pathway,Neuroscience,Neurodegenerative Diseases,Amyloid Plaque and Neurofibrillary Tangle Formation in Alzheimer's Disease,Stem Cells,Cardiovascular,Angiogenesis,MAPK3

Molecular Weight: 38 kDa/40 kDa/43 kDa

Gene ID: 5595

UniProt: P27361

Pathways: MAPK Signaling, RTK Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals, S100 Proteins