Cyclin D1 antibody

Catalog No. ABIN302101

This Mouse Monoclonal antibody specifically detects Cyclin D1 in WB, ELISA, IP, ICC, IHC (p), IHC (fro) and ICFC. It exhibits reactivity toward Human and Rat.

Quick Overview for Cyclin D1 antibody (ABIN302101)

Target: Cyclin D1 (CCND1)

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cyclin D1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Intracellular Flow Cytometry (ICFC)

Clone: CD1-1

Product Details anti-Cyclin D1 Antibody

Purpose: Anti-Cyclin D1 Purified

Specificity: The mouse monoclonal antibody CD1.1 recognizes cyclin D1, an ubiquitously expressed 33 kDa intracellular protein that migrates as a 36 kDa band under reducing SDS-PAGE conditions.

Cross-Reactivity (Details): Human, Rat, Other not determined

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: Purified cyclin D1 protein

Isotype: IgG1

Application Details

Application Notes: Immunohistochemistry (frozen sections): Recommended dilution: 2 μg/mL, positive tissue: colon.
Western blotting: Recommended dilution: 1 μg/mL.
Immunocytochemistry: Recommended dilution: 1 μg/mL.
Immunoprecipitation: Recommended dilution: 1 μg/mL.
Immunohistochemistry (paraffin sections): Heat treatment, sodium citrate buffer pH 6.0.
Flow cytometry: Recommended dilution: 4-10 μg/mL. Intracellular staining.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

Target Details for Cyclin D1

Target: Cyclin D1 (CCND1)

Alternative Name: Cyclin D1

Background: Cyclin D1,Cyclin D1 (PRAD1, Bcl-1) is a cytoplasmic and nuclear protein, which is synthesized during G1 phase and assembles with either cyclin-dependent kinase 4 (CDK4) or CDK6 in response to growth factor stimulation. D-type cyclin-CDK complexes act to inactivate the growth-suppressive function of the Rb protein through its phosphorylation, and titrate CDK inhibitors such as p21Cip1 and p27Kip1. Whereas during G1 phase cyclin D1 accumulates in the nucleus, it translocates into the cytoplasm during S phase. Without growth factor-mediated stimulation, cyclin D1 is unstable, and undergoes ubiquitin-mediated degradation, which is triggered by its phosphorylation. Cyclin D1 destabilization participates in G1/S phase arrest.,CYC-D1, CCND1, Bcl-1, PRAD1, U21B31

Gene ID: 595

UniProt: P24385

Pathways: PI3K-Akt Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, ER-Nucleus Signaling