FZD2 antibody (Internal Region)

Catalog No. ABIN302136

This anti-FZD2 antibody is a Goat Polyclonal antibody detecting FZD2 in WB, ELISA, IF and IHC. Suitable for Human. This Primary Antibody has been cited in 1 publication.

Quick Overview for FZD2 antibody (Internal Region) (ABIN302136)

Target: FZD2 (Frizzled Family Receptor 2 (FZD2))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This FZD2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-FZD2 Antibody

Binding Specificity: Internal Region

Purpose: Frizzled 2

Sequence: RYATLEHPFH CPR

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence RYATLEHPFHCPR, from the internal region of the protein sequence according to NP_001457.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Thyroid Gland shows cytoplasm and membranous staining in epithelial cells of the follicles. Recommended concentration: 2-5 μg/mL. Paraffin embedded Human Liver. Recommended concentration: 2.5 μg/mL.

Western Blot: Approx 60-65 kDa band observed in Human Heart and Placenta lysates (calculated MW of 63.6 kDa according to NP_001457.1) An additional band of approx. 24 kDa was also observed in some lysates which was successfully blocked by incubation with

Peptide ELISA: antibody detection limit dilution 1:64000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the nuclei and cytoplasm of U2OS cells. Recommended concentration: 10μg/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-FZD2 antibody (ABIN302136)

Hirano, Yonezawa, Yunoue, Habu, Uchida, Yoshioka, Kishida, Kishida, Oyoshi, Fujio, Sugata, Yamahata, Hanaya, Arita: "Immunoreactivity of Wnt5a, Fzd2, Fzd6, and Ryk in glioblastoma: evaluative methodology for DAB chromogenic immunostaining." in: Brain tumor pathology, Vol. 31, Issue 2, pp. 85-93, (2014) (PubMed).

Target Details for FZD2

Target: FZD2 (Frizzled Family Receptor 2 (FZD2))

Alternative Name: FZD2

Background: FZD2 , frizzled homolog 2 (Drosophila) , frizzled 2

Gene ID: 2535, 57265, 64512

NCBI Accession: NP_001457

Pathways: WNT Signaling