RAMP1 antibody (C-Term)

Catalog No. ABIN302150

The Goat Polyclonal anti-RAMP1 antibody has been validated for WB and ELISA. It is suitable to detect RAMP1 in samples from Rat.

Quick Overview for RAMP1 antibody (C-Term) (ABIN302150)

Target: RAMP1 (Receptor (G Protein-Coupled) Activity Modifying Protein 1 (RAMP1))

Reactivity: Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This RAMP1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-RAMP1 Antibody

Binding Specificity: C-Term

Purpose: Ramp1 (C-Term., mouse)

Sequence: DYQSKRTEGI V

Specificity: This antibody is expected to recognize isoform 1 (NP_058590.1) only.

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-DYQSKRTEGIV, from the C Terminus of the protein sequence according to NP_058590.1, NP_848488.2.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Experiments gave bands at approx. 18 kDa and 40 kDa in Rat Brain lysate after 0.3 μg/mL antibody staining. The appearance of a band with much higher molecular weight corresponds to earlier findings in literature with different antibodies (Hila

Peptide ELISA: antibody detection limit dilution 1:64000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for RAMP1

Target: RAMP1 (Receptor (G Protein-Coupled) Activity Modifying Protein 1 (RAMP1))

Alternative Name: Ramp1

Background: Ramp1, receptor (calcitonin) activity modifying protein 1, 9130218E19Rik, MGC38240, receptor-activity modifying protein 1

Gene ID: 10267, 51801, 58965

NCBI Accession: NP_058590, NP_848488

Pathways: cAMP Metabolic Process, Myometrial Relaxation and Contraction, Regulation of G-Protein Coupled Receptor Protein Signaling, Regulation of Carbohydrate Metabolic Process