PML antibody (AA 300-600)

Catalog No. ABIN3021659

The Rabbit Polyclonal anti-PML antibody has been validated for WB and IHC. It is suitable to detect PML in samples from Human. There is 1 publication available.

Quick Overview for PML antibody (AA 300-600) (ABIN3021659)

Target: PML (Promyelocytic Leukemia (PML))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PML antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-PML Antibody

Binding Specificity: AA 300-600

Sequence: AVDARYQRDY EEMASRLGRL DAVLQRIRTG SALVQRMKCY ASDQEVLDMH GFLRQALCRL RQEEPQSLQA AVRTDGFDEF KVRLQDLSSC ITQGKDAAVS KKASPEAAST PRDPIDVDLD VSNTTTAQKR KCSQTQCPRK VIKMESEEGK EARLARSSPE QPRPSTSKAV SPPHLDGPPS PRSPVIGSEV FLPNSNHVAS GAGEAEERVV VISSSEDSDA ENSCMEPMET AEPQSSPAHS SPAHSSPAHS SPVQSLLRAQ GASSLPCGTY HPPAWPPHQP AEQAATPDAE PHSEPPDHQE R

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Polyclonal Antibodies

Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 300-600 of human PML (NP_150253.2).

Isotype: IgG

Application Details

Application Notes: WB,1:500 - 1:2000,IHC,1:50 - 1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide,50 % glycerol, pH 7.3.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze / thaw cycles

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

References for anti-PML antibody (ABIN3021659)

Li, Zhong, Liu, Yu, Chen, Wang, Shi, Yuan: "Evidence for Kaposi Sarcoma Originating from Mesenchymal Stem Cell through KSHV-induced Mesenchymal-to-Endothelial Transition." in: Cancer research, Vol. 78, Issue 1, pp. 230-245, (2018) (PubMed).

Target Details for PML

Target: PML (Promyelocytic Leukemia (PML))

Alternative Name: PML

Background: The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This phosphoprotein localizes to nuclear bodies where it functions as a transcription factor and tumor suppressor. Its expression is cell-cycle related and it regulates the p53 response to oncogenic signals. The gene is often involved in the translocation with the retinoic acid receptor alpha gene associated with acute promyelocytic leukemia (APL). Extensive alternative splicing of this gene results in several variations of the protein's central and C-terminal regions, all variants encode the same N-terminus. Alternatively spliced transcript variants encoding different isoforms have been identified.,MYL,PP8675,RNF71,TRIM19,PML,Epigenetics & Nuclear Signaling,Transcription Factors,Cancer,Cell Biology & Developmental Biology,Apoptosis,Ubiquitin,Ubiquitin-Proteasome Signaling Pathway,Endocrine & Metabolism,Cardiovascular,Angiogenesis,PML

Molecular Weight: 47-48 kDa/62-97 kDa

Gene ID: 5371

UniProt: P29590

Pathways: p53 Signaling, Retinoic Acid Receptor Signaling Pathway, Maintenance of Protein Location, Positive Regulation of Endopeptidase Activity, Protein targeting to Nucleus