IL-25 antibody (AA 115-132)

Catalog No. ABIN302245

This Mouse Monoclonal antibody specifically detects IL-25 in WB and IHC (p). It exhibits reactivity toward Human and Mouse.

Quick Overview for IL-25 antibody (AA 115-132) (ABIN302245)

Target: IL-25 (IL25) (Interleukin 25 (IL25))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This IL-25 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 68C1039-2

Product Details anti-IL-25 Antibody

Binding Specificity: AA 115-132

Specificity: This antibody detects Interleukin 17E (IL17E) (IL25).

Cross-Reactivity (Details): Species reactivity (expected):Mouse.
Species reactivity (tested):Human.

Characteristics: Synonyms: IL-25, IL17E, IL-17E, Interleukin-17E

Purification: Protein G Chromatography.

Immunogen: A synthetic peptide of amino acids 115-132, obtained from the human IL-17E proteinsequence.

Isotype: IgG1

Application Details

Application Notes: Western blot (2 μg/mL). Immunohistochemistry on Paraffin Sections (10 μg/mL). Antigen retrieval: Steam slides in 0.01 M sodium citrate buffer, pH 6.0, at 99-100 °C for 20 min. Remove fromheat and let stand at room temperature in buffer for 20 min. Rinse in 1x TBS with Tween(TBST) for 1 min. at room temperature.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: PBS containing 0.05 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or(in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for IL-25

Target: IL-25 (IL25) (Interleukin 25 (IL25))

Alternative Name: Interleukin-25 / IL25

Background: Recently, a number of cytokines belonging to the interleukin (IL)-17 family have been identified. These are termed as IL-17B, IL-17C and IL-17E. IL-17 is a potent proinflammatory cytokine that plays roles in a number of diseases including rheumatoid arthritis, multiple sclerosis, and promotion of tumor growth. IL-17B, C, and E like IL-17 are able to induce proinflammatory responses. However, they do not bind to the IL-17 receptor suggesting that additional IL-17R related receptor might exist. Receptor for IL-17B and IL-17E has been independently isolated by Shi, et al and Lee, et al. and has been designated as EV127 (in mouse) and IL-17Rh1 (in human), respectively. IL-17E induces activation of NF-kB pathway and like IL-17 also induces production of IL-8. The IL17 proteins are a family of potent cytokines that act to induce proinflammatory responses. Studies have shown that IL17E binds strongly to IL17RB. Receptor binding of ligand has been shown to lead to the activation of nuclear factor kappa-B and production of IL8. Exposure of mice to IL17 resulted in a Th-2 like response characterized by increased serum IgE, IgG1 and IgA levels, blood eosinophilia, increased lymphocytes and neutrophils, and pathological changes in the tissues that included eosinophilic infiltrates, increased mucus production, B-lymphocyte hyperplasia and epithelial cell hyperplasia/hypertrophy.Synonyms: IL-17E, IL-25, IL17E, Interleukin-17E

Gene ID: 64806

UniProt: Q9H293