HMGB1 antibody (AA 100-200)

Catalog No. ABIN3023358

This Rabbit Polyclonal antibody specifically detects HMGB1 in WB and IHC. It exhibits reactivity toward Human and has been mentioned in 3+ publications.

Quick Overview for HMGB1 antibody (AA 100-200) (ABIN3023358)

Target: HMGB1 (High Mobility Group Box 1 (HMGB1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HMGB1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-HMGB1 Antibody

Binding Specificity: AA 100-200

Sequence: SAFFLFCSEY RPKIKGEHPG LSIGDVAKKL GEMWNNTAAD DKQPYEKKAA KLKEKYEKDI AAYRAKGKPD AAKKGVVKAE KSKKKKEEEE DEEDEEDEEE E

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Polyclonal Antibodies

Purification: Affinity purification

Immunogen: A synthetic peptide corresponding to a sequence within amino acids 100-200 of human HMGB1 (NP_002119.1).

Isotype: IgG

Application Details

Application Notes: WB,1:500 - 1:2000,IHC,1:50 - 1:200

Restrictions: For Research Use only

Handling

Buffer: PBS with 0.02 % sodium azide,50 % glycerol, pH 7.3.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

References for anti-HMGB1 antibody (ABIN3023358)

Seth, Kimono, Alhasson, Sarkar, Albadrani, Lasley, Horner, Janulewicz, Nagarkatti, Nagarkatti, Sullivan, Chatterjee: "Increased butyrate priming in the gut stalls microbiome associated-gastrointestinal inflammation and hepatic metabolic reprogramming in a mouse model of Gulf War Illness." in: Toxicology and applied pharmacology, Vol. 350, pp. 64-77, (2018) (PubMed).

Yao, Zhao, Tang, Xiong, Zhao, Liu, Dong, Zou, Cai: "Blockade of β-catenin signaling attenuates toluene diisocyanate-induced experimental asthma." in: Allergy, Vol. 72, Issue 4, pp. 579-589, (2017) (PubMed).

Chen, Yu, Yuan, Zhang, Fan, Yuan, Zhang, Yao: "Enriched housing promotes post-stroke functional recovery through astrocytic HMGB1-IL-6-mediated angiogenesis." in: Cell death discovery, Vol. 3, pp. 17054, (2017) (PubMed).

Target Details for HMGB1

Target: HMGB1 (High Mobility Group Box 1 (HMGB1))

Alternative Name: HMGB1

Background: This gene encodes a protein that belongs to the High Mobility Group-box superfamily. The encoded non-histone, nuclear DNA-binding protein regulates transcription, and is involved in organization of DNA. This protein plays a role in several cellular processes, including inflammation, cell differentiation and tumor cell migration. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants that encode the same protein.,HMG-1,HMG1,HMG3,SBP-1,HMGB1,Epigenetics & Nuclear Signaling,Histones,RNA Binding,Cell Biology & Developmental Biology,Apoptosis,HMGB1

Molecular Weight: 24 kDa

Gene ID: 3146

UniProt: P09429

Pathways: p53 Signaling, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration, Inflammasome