CDw60 antibody
Reactivity: Human
IHC (fro), FACS, IF
Host: Mouse
Monoclonal
UM4D4
unconjugated
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- Target
- CDw60
- Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- Un-conjugated
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Application
- Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunofluorescence (IF)
- Characteristics
- This mAb reacts with 9-O-acetylated disialosyl group linked to the glycoprotein CD60w. CDw60 is a carbohydrate antigen present on gangliosides, platelets and a subset of CD4+, CD8+ peripheral T-cells, but not on B-cells, monocytes or granulocytes. CDw60 is involved in the co-stimulation of T-cells.
- Purification
- PEG precipitation
- Immunogen
- The human rheumatoid synovial T cell line (ST-1) was used as the immunogen for the CDw60 antibody.
- Clone
- UM4D4
- Isotype
- IgM
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- Application Notes
- Optimal dilution of the CDw60 antibody should be determined by the researcher.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (Frozen): 0.5-1 μg/mL for 30 min at RT
- Restrictions
- For Research Use only
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- Concentration
- 1 mg/mL
- Buffer
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
- Preservative
- Azide free
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store the CDw60 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- Target
- CDw60
- Background
- This mAb reacts with 9-O-acetylated disialosyl group linked to the glycoprotein CD60w. CDw60 is a carbohydrate antigen present on gangliosides, platelets and a subset of CD4+, CD8+ peripheral T-cells, but not on B-cells, monocytes or granulocytes. CDw60 is involved in the co-stimulation of T-cells.
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