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HSPG2 antibody

HSPG2 Reactivity: Human, Mouse, Cow, Fish, Monkey, Pig IHC (p), IF, FACS Host: Rat Monoclonal A7L6 unconjugated
Catalog No. ABIN3023902
  • Target See all HSPG2 Antibodies
    HSPG2 (Heparan Sulfate Proteoglycan 2 (HSPG2))
    Reactivity
    • 28
    • 17
    • 6
    • 4
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Human, Mouse, Cow, Fish, Monkey, Pig
    Host
    • 19
    • 6
    • 6
    Rat
    Clonality
    • 20
    • 11
    Monoclonal
    Conjugate
    • 21
    • 4
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This HSPG2 antibody is un-conjugated
    Application
    • 20
    • 13
    • 10
    • 9
    • 8
    • 7
    • 5
    • 4
    • 4
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Flow Cytometry (FACS)
    Characteristics
    This mAb specifically precipitates heterogeneous material of high MW, identified as perlecan, a major heparan-sulfate proteoglycan (HSPG) within all basement membranes and cell surfaces. It does not cross-react with laminin, fibronectin, or dermatran sulfate proteoglycan. Because of perlecan s strategic location and ability to store and protect growth factors, it has been strongly implicated in the control of tumor cell growth and metastatic behavior. Perlecan possesses angiogenic and growth-promoting attributes primarily by acting as a co-receptor for basic fibroblast growth factor (FGF-2). Suppression of perlecan causes substantial inhibition of neoplastic growth and neovascularization. Thus, perlecan is a potent inducer of neoplasm growth and angiogenesis in vivo and therapeutic interventions targeting this key modulator of tumor progression may improve neoplastic treatment.
    Purification
    Protein G affinity chromatography
    Immunogen
    A murine EHS laminin preparation was used as the immunogen for the Perlecan antibody.
    Clone
    A7L6
    Isotype
    IgG2a kappa
    Top Product
    Discover our top product HSPG2 Primary Antibody
  • Application Notes
    Optimal dilution of the Perlecan antibody should be determined by the researcher.

    1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
    2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 1-2 μg/mL for 30 min at RT (1),Prediluted format : incubate for 30 min at RT (2)

    Restrictions
    For Research Use only
  • Concentration
    1 mg/mL
    Buffer
    1 mg/mL in 1X PBS, BSA free, sodium azide free
    Preservative
    Azide free
    Storage
    4 °C,-20 °C
    Storage Comment
    Store the Perlecan antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
  • Target
    HSPG2 (Heparan Sulfate Proteoglycan 2 (HSPG2))
    Alternative Name
    Perlecan (HSPG) (HSPG2 Products)
    Synonyms
    HSPG antibody, PLC antibody, PRCAN antibody, SJA antibody, SJS antibody, SJS1 antibody, HSPG2 antibody, AI852380 antibody, Pcn antibody, Plc antibody, per antibody, Per antibody, heparan sulfate proteoglycan 2 antibody, perlecan (heparan sulfate proteoglycan 2) antibody, HSPG2 antibody, hspg2 antibody, Hspg2 antibody
    Background
    This mAb specifically precipitates heterogeneous material of high MW, identified as perlecan, a major heparan-sulfate proteoglycan (HSPG) within all basement membranes and cell surfaces. It does not cross-react with laminin, fibronectin, or dermatran sulfate proteoglycan. Because of perlecan s strategic location and ability to store and protect growth factors, it has been strongly implicated in the control of tumor cell growth and metastatic behavior. Perlecan possesses angiogenic and growth-promoting attributes primarily by acting as a co-receptor for basic fibroblast growth factor (FGF-2). Suppression of perlecan causes substantial inhibition of neoplastic growth and neovascularization. Thus, perlecan is a potent inducer of neoplasm growth and angiogenesis in vivo and therapeutic interventions targeting this key modulator of tumor progression may improve neoplastic treatment.
    Pathways
    Glycosaminoglycan Metabolic Process, Lipid Metabolism
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