Neurofilament antibody

Catalog No. ABIN3023931

The Mouse Monoclonal anti-Neurofilament antibody is suitable to detect Neurofilament in samples from Human. It has been validated for WB and IHC (p).

Quick Overview for Neurofilament antibody (ABIN3023931)

Target: Neurofilament

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Neurofilament antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Grade: Carrier-free

Clone: SPM563

Product Details anti-Neurofilament Antibody

Purpose: Anti-Neurofilament Antibody (azide and preservative free)

Purification: Protein G affinity chromatography

Immunogen: Human recombinant protein was used as the immunogen for this anti-Neurofilament antibody.

Isotype: IgG1, kappa

Application Details

Application Notes: The optimal dilution of the anti-Neurofilament antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the anti-Neurofilament antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for Neurofilament

Target: Neurofilament

Background: This mAb reacts with a 200 kDa protein, identified as heavy sub-unit of neurofilaments (NF-H). Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68 kDa (NF-L), 160 kDa (NF-M) and 200 kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and cell carcinomas of the lung also express neurofilament.

UniProt: P12036