Melanoma gp100 antibody

Catalog No. ABIN3024239

This Mouse Monoclonal antibody specifically detects Melanoma gp100 in IHC (p). It exhibits reactivity toward Human.

Quick Overview for Melanoma gp100 antibody (ABIN3024239)

Target: Melanoma gp100 (PMEL) (Premelanosome Protein (PMEL))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Melanoma gp100 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Grade: Carrier-free

Clone: SPM286

Product Details anti-Melanoma gp100 Antibody

Purpose: Anti-gp100 Antibody / PMEL17 (azide and preservative free)

No Cross-Reactivity: Rat (Rattus)

Purification: Protein G affinity chromatography

Immunogen: Membranes from a human melanoma metastasis were used as the immunogen for this anti-gp100 antibody.

Isotype: IgG2b, kappa

Application Details

Application Notes: The optimal dilution of the anti-gp100 antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the anti-gp100 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for Melanoma gp100

Target: Melanoma gp100 (PMEL) (Premelanosome Protein (PMEL))

Alternative Name: gp100

Background: By immunohistochemistry, it specifically recognizes a protein in melanocytes and melanomas. This mAb reacts with junctional and blue nevus cells and variably with fetal and neonatal melanocytes. Intradermal nevi, normal adult melanocytes, and non-melanocytic cells are negative. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.This Mab labels formalin-fixed, paraffin-embedded melanomas and other tumors showing melanocytic differentiation.

UniProt: P40967