CD2 antibody

Catalog No. ABIN3024312

The Mouse Monoclonal anti-CD2 antibody is suitable to detect CD2 in samples from Human and Cat. It has been validated for FACS, IF, WB and Func.

Quick Overview for CD2 antibody (ABIN3024312)

Target: CD2

Reactivity: Human, Cat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD2 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB), Functional Studies (Func)

Clone: HuLy-m1

Product Details anti-CD2 Antibody

Purification: Protein G affinity chromatography

Immunogen: Human thymocytes were used as the immunogen for the CD2 antibody.

Isotype: IgG2b kappa

Application Details

Application Notes: Optimal dilution of the CD2 antibody should be determined by the researcher.\. Functional Activity (Order SAF formulation),Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL, Western blot: 1-2 μg/mL

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the CD2 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for CD2

Target: CD2

Alternative Name: CD2

Background: CD2 interacts through its amino-terminal domain with the extracellular domain of CD58 (also designated CD2 ligand) to mediate cell adhesion. CD2/CD58 binding can enhance antigen-specific T cell activation. CD2 is a transmembrane glycoprotein that is expressed on peripheral blood T lymphocytes, NK cells and thymocytes. CD58 is a heavily glycosylated protein with a broad tissue distribution in hematopoietic and other cells, including endothelium. Interaction between CD2 and its counter receptor LFA3 (CD58) on opposing cells optimizes immune system recognition, thereby facilitating communication between helper T lymphocytes and antigen-presenting cells, as well as between cytolytic effectors and target cells.