CD45RB (CD45RB) antibody

Details for Product No. ABIN3024427
Target
Reactivity
Human
124
39
11
6
5
4
3
1
Host
Mouse
107
39
18
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
16
12
12
8
5
5
5
5
5
5
5
5
5
5
5
5
5
2
2
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
154
93
79
47
31
27
16
12
3
3
2
1
Options
Immunogen Human peripheral blood lymphocytes maintained in T cell growth factor were used as the immunogen for this CD45RB antibody.
Clone PD7-26
Isotype IgG1, kappa
Purification Protein G purified
Target
Alternative Name CD45RB (CD45RB Antibody Abstract)
Background CD45RB is one of the four major isoforms of CD45.  The variation in these isoforms is localized to the extracellular domain of CD45R, while the intracellular domain is conserved.  Clone PD7/26 antibody is specific to CD45RB, and stains most leukemias and lymphomas. This CD45RB antibody is often used in a cocktail with clone 2B11, which will bind to all CD45 isoforms (< a href=../cd45-antibody-cocktail-2b11-pd726-v2243>Cat No V2243).
Gene ID 5788
Application Notes Variations in protocols, secondaries and substrates may require the CD45RB antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Western Blot: 0.5-1 μg/mL, FACS: 0.5-1 μg/million cells,IF: 0.5-1 μg/mL,IHC (FFPE): 0.5-1.0 μg/mL for 30 minutes at RT (1),Prediluted format : incubate for 30 min at RT (2)

Restrictions For Research Use only
Concentration 1 mg/mL
Buffer 1 mg/mL in 1X PBS, BSA free, sodium azide free
Preservative Azide free
Storage 4 °C,-20 °C
Storage Comment Store the CD45RB antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Images
Image no. 1 for anti-CD45RB (CD45RB) antibody (ABIN3024427) Western blot testing of Daudi cell lysate with CD45RB antibody (clone PD7/26). Predic...
Image no. 2 for anti-CD45RB (CD45RB) antibody (ABIN3024427) SDS-PAGE Analysis of Purified, BSA-Free CD45RB Antibody (clone PD7/26). Confirmation ...
Image no. 3 for anti-CD45RB (CD45RB) antibody (ABIN3024427) IHC testing of human tonsil (10X) stained with CD45RB antibody (PD7/26).