CD45RB antibody

Catalog No. ABIN3024427

The Mouse Monoclonal anti-CD45RB antibody (Clone PD7-26) (ABIN3024427) specifically detects CD45RB in FACS, WB, IF and IHC (p). The antibody is reactive with Human samples.

Quick Overview for CD45RB antibody (ABIN3024427)

Target: CD45RB

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD45RB antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Grade: Carrier-free

Clone: PD7-26

Product Details anti-CD45RB Antibody

Purpose: CD45RB Antibody (azide and preservative free)

Purification: Protein G purified

Immunogen: Human peripheral blood lymphocytes maintained in T cell growth factor were used as the immunogen for this CD45RB antibody.

Isotype: IgG1, kappa

Application Details

Application Notes: Variations in protocols, secondaries and substrates may require the CD45RB antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the CD45RB antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for CD45RB

Target: CD45RB

Alternative Name: CD45RB

Background: CD45RB is one of the four major isoforms of CD45.  The variation in these isoforms is localized to the extracellular domain of CD45R, while the intracellular domain is conserved.  Clone PD7/26 antibody is specific to CD45RB, and stains most leukemias and lymphomas. This CD45RB antibody is often used in a cocktail with clone 2B11, which will bind to all CD45 isoforms (< a href=../cd45-antibody-cocktail-2b11-pd726-v2243>Cat No V2243).