CD70 antibody

Catalog No. ABIN3024471

The Mouse Monoclonal anti-CD70 antibody is suitable to detect CD70 in samples from Human. It has been validated for ELISA, FACS, IP, IF, IHC (fro) and InhA.

Quick Overview for CD70 antibody (ABIN3024471)

Target: CD70 (CD70 Molecule (CD70))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD70 antibody is un-conjugated

Application: ELISA, Flow Cytometry (FACS), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Inhibition Assay (InhA)

Clone: BU69

Product Details anti-CD70 Antibody

Purification: Protein G affinity chromatography

Immunogen: The human WM-1 (Waldenstrom s macroglobulinemia) cell line was used as the immunogen for the CD70 antibody.

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal dilution of the CD70 antibody should be determined by the researcher.

1. This mAb blocks the interaction between CD27 and CD70, and has been shown to inhibit T cell proliferation induced by dendritic cells.\. ELISA (order Ab without BSA),Functional Activity: Inhibits T cell proliferation induced by dendritic cells (Order SAF formulation) (1),Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunoprecipitation: 0.5-1 μg/500 μg protein lysate,Immunohistochemistry (Frozen): 0.5-1 μg/mL for 30 min at RT

Restrictions: For Research Use only

Handling

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the CD70 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for CD70

Target: CD70 (CD70 Molecule (CD70))

Alternative Name: CD70

Background: CD70 belongs to the tumor necrosis factor (TNF) ligand family. It is a cytokine that binds to CD27. Plays a role in T-cell activation. Induces the proliferation of costimulated T-cells and enhances the generation of cytolytic T-cells.

Pathways: Cancer Immune Checkpoints