Cyclin D1 antibody (full length)

Catalog No. ABIN3024574

The Mouse Monoclonal anti-Cyclin D1 antibody (Clone SPM587) (ABIN3024574) specifically detects Cyclin D1 in WB, FACS and IHC (p). The antibody is reactive with Human and Mouse samples.

Quick Overview for Cyclin D1 antibody (full length) (ABIN3024574)

Target: Cyclin D1 (CCND1)

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cyclin D1 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: SPM587

Product Details anti-Cyclin D1 Antibody

Binding Specificity: full length

Purpose: CCND1 Antibody

Purification: Protein G affinity chromatography

Immunogen: Recombinant full length human protein was used as the immunogen for this CCND1 antibody.

Isotype: IgG2a, kappa

Application Details

Application Notes: The optimal dilution of the CCND1 antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the CCND1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for Cyclin D1

Target: Cyclin D1 (CCND1)

Alternative Name: CCND1

Background: Cyclin D1 (CCND1), one of the key cell cycle regulators, is a putative proto-oncogene overexpressed in a wide variety of human neoplasms. About 60 % of mantle cell lymphomas (MCL) contain a t(11, 14)(q13, q32) translocation resulting in over-expression of Cyclin D1. This antibody is useful in identifying mantle cell lymphomas (CCND1+) from CLL/SLL and follicular lymphomas (CCND1-). Occasionally, hairy cell leukemia and plasma cell myeloma weakly express CCND1.

UniProt: P24385

Pathways: PI3K-Akt Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, ER-Nucleus Signaling