KRT8 antibody

Catalog No. ABIN3024648

The Mouse Monoclonal anti-KRT8 antibody has been validated for IF, FACS and IHC (p). It is suitable to detect KRT8 in samples from Human, Cow, Rabbit, Pig and Sheep.

Quick Overview for KRT8 antibody (ABIN3024648)

Target: KRT8 (Keratin 8 (KRT8))

Reactivity: Human, Cow, Rabbit, Pig, Sheep

Host: Mouse

Clonality: Monoclonal

Conjugate: This KRT8 antibody is un-conjugated

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: B22-1

Product Details anti-KRT8 Antibody

No Cross-Reactivity: Mouse (Murine), Rat (Rattus), Chicken, Xenopus laevis

Purification: Protein G affinity chromatography

Immunogen: A cytoskeletal preparation from HeLa cells was used as the immunogen for the Cytokeratin 8 antibody.

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal dilution of the Cytokeratin 8 antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format : incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the Cytokeratin 8 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for KRT8

Target: KRT8 (Keratin 8 (KRT8))

Alternative Name: Cytokeratin 8

Background: Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). CK8 is primarily found in the non-squamous epithelia and is present in majority of adenocarcinomas and ductal carcinomas. It is absent in squamous cell carcinomas. Hepatocellular carcinomas are defined by the use of antibodies that recognize only cytokeratin 8 and 18. CK8 exists on several types of normal and neoplastic epithelia, including many ductal and glandular epithelia such as colon, stomach, small intestine, trachea, and esophagus as well as in transitional epithelium. Anti-CK8 does not react with skeletal muscle or nerve cells. Epithelioid sarcoma, chordoma, and adamantinoma show strong positivity corresponding to that of simple epithelia (with antibodies against CK8, CK18 and CK19). Reportedly, anti-CK8 is useful for the differentiation of lobular (ring-like, perinuclear) from ductal (peripheral-predominant) carcinoma of the breast.