FBXO5 antibody (AA 1-250)

Catalog No. ABIN3024717

The Mouse Monoclonal anti-FBXO5 antibody has been validated for WB, IF, FACS and IHC (p). It is suitable to detect FBXO5 in samples from Human.

Quick Overview for FBXO5 antibody (AA 1-250) (ABIN3024717)

Target: FBXO5 (F-Box Protein 5 (FBXO5))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This FBXO5 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: EMI1-1176

Product Details anti-FBXO5 Antibody

Binding Specificity: AA 1-250

Purification: Protein G affinity chromatography

Immunogen: A recombinant fragment (203 amino acid residues between aa 1-250) from the human protein was used as the immunogen for the EMI1 antibody.

Isotype: IgG2a kappa

Application Details

Application Notes: Optimal dilution of the EMI1 antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0 for 10-20 min followed by cooling at RT for 20 minutes
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Western blot: 1-2 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1),Prediluted format : incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the EMI1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for FBXO5

Target: FBXO5 (F-Box Protein 5 (FBXO5))

Alternative Name: EMI1

Background: Early Mitotic Inhibitor-1 regulates mitosis by inhibiting the anaphase promoting complex/cyclosome (APC). It is a conserved F box protein containing a zinc-binding region essential for APC inhibition. The protein functions to promote cyclin A accumulation and S phase entry in somatic cells by inhibiting the APC complex. At the G1-S transition, EMI1 is transcriptionally induced by the E2F transcription factor. Overexpression accelerates S-phase entry and can override a G1 block caused by overexpression of Cdh1 or the E2F-inhibitor p105 retinoblastoma protein (pRb). Depleting cells of EMI1 through RNA interference prevents accumulation of cyclin A and inhibits S phase entry.

Pathways: Mitotic G1-G1/S Phases