Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

FBXO5 antibody (AA 1-250)

FBXO5 Reactivity: Human WB, IF, FACS, IHC (p) Host: Mouse Monoclonal EMI1-1176 unconjugated
Catalog No. ABIN3024717
  • Target See all FBXO5 Antibodies
    FBXO5 (F-Box Protein 5 (FBXO5))
    Binding Specificity
    • 11
    • 7
    • 7
    • 6
    • 6
    • 5
    • 2
    • 1
    • 1
    • 1
    AA 1-250
    Reactivity
    • 44
    • 9
    • 9
    • 3
    • 2
    • 1
    • 1
    Human
    Host
    • 27
    • 17
    Mouse
    Clonality
    • 24
    • 20
    Monoclonal
    Conjugate
    • 20
    • 4
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This FBXO5 antibody is un-conjugated
    Application
    • 35
    • 17
    • 12
    • 9
    • 8
    • 6
    • 5
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Purification
    Protein G affinity chromatography
    Immunogen
    A recombinant fragment (203 amino acid residues between aa 1-250) from the human protein was used as the immunogen for the EMI1 antibody.
    Clone
    EMI1-1176
    Isotype
    IgG2a kappa
    Top Product
    Discover our top product FBXO5 Primary Antibody
  • Application Notes
    Optimal dilution of the EMI1 antibody should be determined by the researcher.

    1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0 for 10-20 min followed by cooling at RT for 20 minutes
    2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Western blot: 1-2 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1),Prediluted format : incubate for 30 min at RT (2)

    Restrictions
    For Research Use only
  • Concentration
    1 mg/mL
    Buffer
    1 mg/mL in 1X PBS, BSA free, sodium azide free
    Preservative
    Azide free
    Storage
    4 °C,-20 °C
    Storage Comment
    Store the EMI1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
  • Target
    FBXO5 (F-Box Protein 5 (FBXO5))
    Alternative Name
    EMI1 (FBXO5 Products)
    Synonyms
    EMI1 antibody, FBX5 antibody, Fbxo31 antibody, 2510044I10Rik antibody, C85305 antibody, Emi1 antibody, emi1 antibody, fc65h02 antibody, wu:fc65h02 antibody, wu:fe06e07 antibody, wu:fz79f03 antibody, zgc:136397 antibody, zgc:158541 antibody, fbx5 antibody, Fbxo5 antibody, ACYPI007854 antibody, fbxo5 antibody, F-box protein 5 antibody, F-box only protein 5 antibody, F-box protein 5 L homeolog antibody, F-box protein 5 S homeolog antibody, FBXO5 antibody, Fbxo5 antibody, fbxo5 antibody, fbxo5.L antibody, fbxo5.S antibody
    Background
    Early Mitotic Inhibitor-1 regulates mitosis by inhibiting the anaphase promoting complex/cyclosome (APC). It is a conserved F box protein containing a zinc-binding region essential for APC inhibition. The protein functions to promote cyclin A accumulation and S phase entry in somatic cells by inhibiting the APC complex. At the G1-S transition, EMI1 is transcriptionally induced by the E2F transcription factor. Overexpression accelerates S-phase entry and can override a G1 block caused by overexpression of Cdh1 or the E2F-inhibitor p105 retinoblastoma protein (pRb). Depleting cells of EMI1 through RNA interference prevents accumulation of cyclin A and inhibits S phase entry.
    Pathways
    Mitotic G1-G1/S Phases
You are here:
Support