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Glypican 3 antibody

GPC3 Reactivity: Human, Rat IF, FACS, IHC (p) Host: Mouse Monoclonal GPC3-863 unconjugated
Catalog No. ABIN3024878
  • Target See all Glypican 3 (GPC3) Antibodies
    Glypican 3 (GPC3)
    Reactivity
    • 153
    • 48
    • 48
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Human, Rat
    Host
    • 97
    • 55
    • 2
    Mouse
    Clonality
    • 82
    • 71
    • 1
    Monoclonal
    Conjugate
    • 64
    • 13
    • 8
    • 7
    • 7
    • 6
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    This Glypican 3 antibody is un-conjugated
    Application
    • 83
    • 82
    • 71
    • 51
    • 41
    • 26
    • 18
    • 17
    • 6
    • 2
    • 2
    • 1
    • 1
    Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Purification
    Protein G affinity chromatography
    Immunogen
    Recombinant full-length human GPC3 protein was used as the immunogen for the Glypican-3 antibody.
    Clone
    GPC3-863
    Isotype
    IgG1 kappa
    Top Product
    Discover our top product GPC3 Primary Antibody
  • Application Notes
    Optimal dilution of the Glypican-3 antibody should be determined by the researcher.

    1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Tris with 1  mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 min
    2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format : incubate for 30 min at RT (2)

    Restrictions
    For Research Use only
  • Concentration
    0.2 mg/mL
    Buffer
    0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Store the Glypican-3 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
  • Target
    Glypican 3 (GPC3)
    Alternative Name
    Glypican-3 (GPC3 Products)
    Synonyms
    GPC3 antibody, sgb antibody, dgsx antibody, sdys antibody, sgbs antibody, oci-5 antibody, sgbs1 antibody, DGSX antibody, GTR2-2 antibody, MXR7 antibody, OCI-5 antibody, SDYS antibody, SGB antibody, SGBS antibody, SGBS1 antibody, Glypican-3 antibody, glypican 3 antibody, gpc3 antibody, GPC3 antibody, Gpc3 antibody
    Background
    Glypican-3 (GPC3) is a glycosylphospatidyl inositol-anchored membrane protein, which may also be found in a secreted form. Anti-GPC3 has been identified as a useful tumor marker for the diagnosis of hepatocellular carcinoma (HCC), hepatoblastoma, melanoma, testicular germ cell tumors, and Wilm s tumor. In patients with HCC, GPC3 is overexpressed in neoplastic liver tissue and elevated in serum, but is undetectable in normal liver, benign liver, and the serum of healthy donors. GPC3 expression is also found to be higher in HCC liver tissue than in cirrhotic liver or liver with focal lesions such as dysplastic nodules and areas of hepatic adenoma (HA) with malignant transformation. In the context of testicular germ cell tumors, GPC3 expression is up regulated in certain histologic subtypes, specifically yolk sac tumors and choriocarcinoma. A high level of GPC3 expression is also found in some types of embryonal tumors, such as Wilm s tumor and hepatoblastoma, with a low or undetectable expression in normal adjacent tissue. In patients with thyroid cancer, expression of GPC3 is dramatically enhanced in certain types of cancers: 100 % in follicular carcinoma and 70 % in papillary carcinoma. Expression of GPC3 in follicular carcinoma is significantly higher than that of follicular adenoma. In contrast, GPC3 is not expressed in anaplastic carcinoma.
    Pathways
    Glycosaminoglycan Metabolic Process
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