TOX3 antibody (AA 200-400)

Catalog No. ABIN3025030

Product Details anti-TOX3 Antibody

Target: TOX3 (TOX High Mobility Group Box Family Member 3 (TOX3))

Binding Specificity: AA 200-400

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TOX3 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Characteristics: It recognizes a 63 kDa protein, which is identified as TOX3. It contains a high mobility group (HMG)-box, which regulates Ca2+-dependent transcription in neurons through interaction with the cAMP-response-element-binding protein (CREB). TOX3 appears to be associated with breast cancer susceptibility and is expressed downstream of a cytoprotective cascade together with CITED1, a transcriptional regulator that does not bind directly to DNA. TOX3 is predominantly expressed in the brain and forms homodimers. TOX3 overexpression protects neuronal cells from cell death caused by endoplasmic reticulum stress or BAX overexpression through the induction of anti-apoptotic transcripts and repression of pro-apoptotic transcripts.

Purification: Protein G affinity chromatography

Immunogen: A recombinant fragment (139 amino acid residues around aa 200-400) from the human protein was used as the immunogen for the TOX3 antibody.

Clone: TOX3-1123

Isotype: IgG2b kappa

Application Details

Application Notes: Optimal dilution of the TOX3 antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Tris with 1  mM EDTA,  pH 9.0 or 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format : incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the TOX3 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for TOX3

Target: TOX3 (TOX High Mobility Group Box Family Member 3 (TOX3))

Alternative Name: TOX3 (TOX3 Products)

Synonyms: TNRC9 antibody, cagf9 antibody, tnrc9 antibody, CAGF9 antibody, 500-9 antibody, BC052044 antibody, C230068E13 antibody, Tnrc9 antibody, TOX high mobility group box family member 3 antibody, TOX3 antibody, tox3 antibody, Tox3 antibody

Background: It recognizes a 63  kDa protein, which is identified as TOX3. It contains a high mobility group (HMG)-box, which regulates Ca2+-dependent transcription in neurons through interaction with the cAMP-response-element-binding protein (CREB). TOX3 appears to be associated with breast cancer susceptibility and is expressed downstream of a cytoprotective cascade together with CITED1, a transcriptional regulator that does not bind directly to DNA. TOX3 is predominantly expressed in the brain and forms homodimers. TOX3 overexpression protects neuronal cells from cell death caused by endoplasmic reticulum stress or BAX overexpression through the induction of anti-apoptotic transcripts and repression of pro-apoptotic transcripts.

Pathways: Chromatin Binding