TRIM29 antibody (AA 1-200)

Catalog No. ABIN3025033

This anti-TRIM29 antibody is a Mouse Monoclonal antibody detecting TRIM29 in IF, FACS and IHC (p). Suitable for Human.

Quick Overview for TRIM29 antibody (AA 1-200) (ABIN3025033)

Target: TRIM29 (Tripartite Motif Containing 29 (TRIM29))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TRIM29 antibody is un-conjugated

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: TRIM29-1041

Product Details anti-TRIM29 Antibody

Binding Specificity: AA 1-200

Characteristics: It recognizes a 66 kDa protein, which is identified as Tripartite motif-containing protein 29 (TRIM29). It interacts with the intermediate filament protein vimentin, a substrate for the PKC family of protein kinases, and with hPKCI-1, an inhibitor of the PKCs. TRIM29 protein contains both zinc finger and leucine zipper motifs, suggesting that the it may form homodimers and possibly associate with DNA. High expression of TRIM29 has been reported in gastric cancer and pancreatic cancer, and correlates with enhanced tumor growth and lymph node metastasis. TRIM29 is also able to distinguish lung squamous cell carcinoma from lung adenocarcinoma with ~90 % positive accuracy, when used in a panel with TTF-1, p63, CK5/6, and Napsin-A antibodies.

Purification: Protein G affinity chromatography

Immunogen: A recombinant fragment (126 amino acid residues between aa 1-200) of human TRIM29 protein was used as the immunogen for the TRIM29 antibody.

Isotype: IgG2a kappa

Application Details

Application Notes: Optimal dilution of the TRIM29 antibody should be determined by the researcher.

1. Staining of formalin/paraffin tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format : incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the TRIM29 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for TRIM29

Target: TRIM29 (Tripartite Motif Containing 29 (TRIM29))

Alternative Name: TRIM29

Background: It recognizes a 66  kDa protein, which is identified as Tripartite motif-containing protein 29 (TRIM29). It interacts with the intermediate filament protein vimentin, a substrate for the PKC family of protein kinases, and with hPKCI-1, an inhibitor of the PKCs. TRIM29 protein contains both zinc finger and leucine zipper motifs, suggesting that the it may form homodimers and possibly associate with DNA. High expression of TRIM29 has been reported in gastric cancer and pancreatic cancer, and correlates with enhanced tumor growth and lymph node metastasis. TRIM29 is also able to distinguish lung squamous cell carcinoma from lung adenocarcinoma with ~90 % positive accuracy, when used in a panel with TTF-1, p63, CK5/6, and Napsin-A antibodies.

Pathways: p53 Signaling, Apoptosis, DNA Damage Repair, Inositol Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus