Biotin antibody

Catalog No. ABIN3025157

This Mouse Monoclonal antibody specifically detects Biotin in WB, FACS, IF, CM, IHC (p) and ISH.

Quick Overview for Biotin antibody (ABIN3025157)

Target: Biotin

Host: Mouse

Clonality: Monoclonal

Conjugate: This Biotin antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Confocal Microscopy (CM), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), In situ hybridization (ISH)

Clone: SPM375

Product Details anti-Biotin Antibody

Characteristics: It recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This mAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.

Purification: Protein G affinity chromatography

Immunogen: Biotinylated sheep immunoglobulin was used as the immunogen for this anti-Biotin antibody.

Isotype: IgG1

Application Details

Application Notes: The optimal dilution of the anti-Biotin antibody for each application should be determined by the researcher.

1. No special pretreatment is required for the immunohistochemical staining of formalin-fixed, paraffin-embedded tissues.\. Confocal Microscopy,Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,In situ Nucleic Acid Hybridization,Western: blot 1-3 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1)

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the anti-Biotin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for Biotin

Target: Biotin

Target Type: Chemical

Background: It recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This mAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.