Biotin antibody

Catalog No. ABIN3025157

This Mouse Monoclonal antibody specifically detects Biotin in WB, FACS, IF and IHC (p).

Quick Overview for Biotin antibody (ABIN3025157)

Target: Biotin

Host: Mouse

Clonality: Monoclonal

Conjugate: This Biotin antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Grade: Carrier-free

Clone: SPM375

Product Details anti-Biotin Antibody

Purpose: Anti-Biotin Antibody (azide and preservative free)

Characteristics: It recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This mAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.

Purification: Protein G affinity chromatography

Immunogen: Biotinylated sheep immunoglobulin was used as the immunogen for this anti-Biotin antibody.

Isotype: IgG1, kappa

Application Details

Application Notes: The optimal dilution of the anti-Biotin antibody for each application should be determined by the researcher.

1. No special pretreatment is required for the immunohistochemical staining of formalin-fixed, paraffin-embedded tissues.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the anti-Biotin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for Biotin

Target: Biotin

Target Type: Chemical

Background: It recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This mAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.