GFM1 antibody

Catalog No. ABIN3025203

The Mouse Monoclonal anti-GFM1 antibody has been validated for WB, IF, IHC (p) and FACS. It is suitable to detect GFM1 in samples from Human.

Quick Overview for GFM1 antibody (ABIN3025203)

Target: GFM1 (G-Elongation Factor, Mitochondrial 1 (GFM1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This GFM1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

Clone: MTC719

Product Details anti-GFM1 Antibody

No Cross-Reactivity: Insect, Bacteria

Characteristics: MAb MTC719 recognizes a 60 kDa antigen associated with the mitochondria in cells. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. MAb MTC719 recognizes an antigen associated with the mitochondria in cells from a wide variety of animals, but not insects and bacteria. It can be used to stain the mitochondria in cell or tissue preparations and can be used as a mitochondrial marker in subcellular fractions. It produces a spaghetti-like pattern in normal and malignant cells and may be used to stain mitochondria of cells in fixed or frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations.

Purification: Protein G affinity chromatography

Immunogen: The Mitochondrial fraction of HeLa cells was used as the immunogen for this Mitochondrial antibody.

Isotype: IgG1 kappa

Application Details

Application Notes: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Mitochondrial antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10  mM Citrate Buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. FACS: 0.5-1 μg/million cells,IF: 0.5-1 μg/mL,WB: 0.25-0.5 μg/mL,IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1),Prediluted format : incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the Mitochondrial antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for GFM1

Target: GFM1 (G-Elongation Factor, Mitochondrial 1 (GFM1))

Alternative Name: Mitochondrial

Background: MAb MTC719 recognizes a 60  kDa antigen associated with the mitochondria in cells. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. MAb MTC719 recognizes an antigen associated with the mitochondria in cells from a wide variety of animals, but not insects and bacteria. It can be used to stain the mitochondria in cell or tissue preparations and can be used as a mitochondrial marker in subcellular fractions. It produces a spaghetti-like pattern in normal and malignant cells and may be used to stain mitochondria of cells in fixed or frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations.