PDE8A antibody (N-Term)

Catalog No. ABIN302523

The Rabbit Polyclonal anti-PDE8A antibody (ABIN302523) specifically detects PDE8A in IHC (p). The antibody is reactive with Human samples.

Quick Overview for PDE8A antibody (N-Term) (ABIN302523)

Target: PDE8A (phosphodiesterase 8A (PDE8A))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PDE8A antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PDE8A Antibody

Binding Specificity: N-Term

Specificity: This antibody detects Phosphodiesterase 8A (PDE8A) near N-terminus.

Purification: Immunoaffinity chromatography

Immunogen: PDE8A antibody was raised against synthetic peptide from near N-terminus of human PDE8A.

Application Details

Application Notes: Immunohistochemistry on paraffin sections (10 μg/mL). Antigen retrieval: Steam slides in 0.01 M sodium citrate buffer, pH 6.0, at 99-100C for 20 min. Remove from heat and let stand at room temperature in buffer for 20 min. Rinse in 1xTBS with Tween (TBST) for 1 min. at room temperature.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS containing 0.09 % sodium azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C to -70 °C for longer.

Target Details for PDE8A

Target: PDE8A (phosphodiesterase 8A (PDE8A))

Alternative Name: PDE8A

Background: Phosphodiesterase 8A, a high affinity, cAMP-specific phosphodiesterase, has been implicated in penile erectile dysfunction. PDE8A activity is inhibited by dipyridamole, but not by rolipram, zaprinast, or IBMX. At least five splice variants and four protein isoforms have been reported.Synonyms: 5'-cyclic phosphodiesterase 8A, FLJ16150, High affinity cAMP-specific and IBMX-insensitive 3', HsT19550, Phosphodiesterase 8A, cAMP-specific cyclic nucleotide phosphodiesterase 8A

Gene ID: 5151

NCBI Accession: NP_002596

UniProt: O60658

Pathways: cAMP Metabolic Process