Myogenin antibody (full length)

Catalog No. ABIN3025312

The Mouse Monoclonal anti-Myogenin antibody (Clone MGN185-F5D) (ABIN3025312) specifically detects Myogenin in IHC (p). The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for Myogenin antibody (full length) (ABIN3025312)

Target: Myogenin (MYOG) (Myogenin (Myogenic Factor 4) (MYOG))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Myogenin antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: MGN185-F5D

Product Details anti-Myogenin Antibody

Binding Specificity: AA 30-224, full length

Purpose: Myogenin Antibody Cocktail

Purification: Protein G affinity chromatography

Immunogen: Full length human recombinant protein (MGN185) and a partial rat recombinant protein (amino acid 30-224) (F5D) were used as the immunogens for this Myogenin antibody cocktail.

Isotype: IgG1, kappa

Application Details

Application Notes: The optimal dilution of the Myogenin antibody for each application should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the Myogenin antibody cocktail at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for Myogenin

Target: Myogenin (MYOG) (Myogenin (Myogenic Factor 4) (MYOG))

Alternative Name: Myogenin

Background: Myogenin is a member of the MyoD family of myogenic basic helix-loop-helix (bHLH) transcription factors that also includes MyoD, Myf-5, and MRF4 (also known as herculinor Myf-6). MyoD family members are expressed exclusively in skeletal muscle and play a key role in activating myogenesis by binding to enhancer sequences of muscle-specific genes. The regulatory domain of MyoD is approximately 70 amino acids in length and includes both a basic DNA binding motif and a bHLH dimerization motif. MyoD family members share about 80 % amino acid homology in their bHLH motifs. Anti-myogenin labels the nuclei of myoblasts in developing muscle tissue, and is expressed in tumor cell nuclei of rhabdomyosarcoma and some leiomyosarcomas. Positive nuclear staining may occur in Wilms tumor.

UniProt: P15173

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development