ACTH antibody (AA 1-24)
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- Target See all ACTH Antibodies
- ACTH (Adrenocorticotropic hormone (ACTH))
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Binding Specificity
- AA 1-24
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Reactivity
- Human, Rat, Mouse
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This ACTH antibody is un-conjugated
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Application
- Immunofluorescence (IF), Flow Cytometry (FACS), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Purification
- Protein G affinity chromatography
- Immunogen
- A synthetic peptide corresponding to aa 1-24 of human ACTH was used as the immunogen for this antibody.
- Clone
- 2F6
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product ACTH Primary Antibody
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- Application Notes
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The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.
1. No special pretreatment is required for staining of formalin-fixed tissues.\. ELISA: order BSA-free format for coating,FACS: 0.5-1 μg/million cells,IF: 0.5-1 μg/mL,IHC (FFPE): 0.5-1.0 μg/mL for 30 minutes at RT (1)
- Restrictions
- For Research Use only
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- Concentration
- 1 mg/mL
- Buffer
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
- Preservative
- Azide free
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store the ACTH antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- Target
- ACTH (Adrenocorticotropic hormone (ACTH))
- Alternative Name
- ACTH / Synacthen (ACTH Products)
- Synonyms
- ACTH antibody, BE antibody, Beta-LPH antibody, Clip antibody, Gamma-LPH antibody, Npp antibody, Pomc-1 antibody, Pomc1 antibody, alpha-MSH antibody, alphaMSH antibody, beta-MSH antibody, gamma-MSH antibody, CLIP antibody, LPH antibody, MSH antibody, NPP antibody, POC antibody, pro-opiomelanocortin-alpha antibody, proopiomelanocortin antibody, Pomc antibody, POMC antibody
- Target Type
- Hormone
- Background
- POMC (pro-opiomelanocortin or corticotropin-lipotropin) is a 267 amino acid polypeptide hormone precursor that goes through extensive, tissue-specific post-translational processing by convertases. POMC is cleaved into ten hormone chains named NPP, ACTH, alpha-MSH (Melanocyte Stimulating Hormone), beta-MSH, gamma-MSH, CLIP (corticotropin-like intermediary peptide), Lipotropin-beta, Lipotropin-gamma, beta-endorphin and Met-enkephalin. ACTH (also called Corticotropin) is a 39 amino acid active peptide produced by the anterior pituitary. This antibody is specific to Synacthen (aa 1-24 of ACTH) and does not react with CLIP (aa 17-39 of ACTH). ACTH is produced by cells of immune system (T-cells, B-cells, and macrophages) in response to stimuli associated with stress. ACTH antibody is a useful marker in classification of pituitary tumors and the study of pituitary disease. It reacts with ACTH-producing cells (corticotrophs). It also may react with other tumors (e.g. some small cell carcinomas of the lung) causing paraneoplastic syndromes by secreting ACTH.
- Gene ID
- 5443
- Pathways
- Metabolism of Steroid Hormones and Vitamin D, Peptide Hormone Metabolism, Hormone Activity
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