Transglutaminase 2 antibody (full length)

Catalog No. ABIN3025598

This Mouse Monoclonal antibody specifically detects Transglutaminase 2 in IHC (p). It exhibits reactivity toward Human.

Quick Overview for Transglutaminase 2 antibody (full length) (ABIN3025598)

Target: Transglutaminase 2 (TGM2) (Transglutaminase 2 (C Polypeptide, Protein-Glutamine-gamma-Glutamyltransferase) (TGM2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Transglutaminase 2 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Grade: Carrier-free

Clone: TGM2-419

Product Details anti-Transglutaminase 2 Antibody

Binding Specificity: full length

Purpose: Transglutaminase 2 Antibody (azide and preservative free)

Purification: Protein G affinity chromatography

Immunogen: Recombinant full-length human protein was used as the immunogen for the Transglutaminase 2 antibody.

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal dilution of the Transglutaminase 2 antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the Transglutaminase 2 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for Transglutaminase 2

Target: Transglutaminase 2 (TGM2) (Transglutaminase 2 (C Polypeptide, Protein-Glutamine-gamma-Glutamyltransferase) (TGM2))

Alternative Name: Transglutaminase 2

Background: Recognizes a 77-85 kDa protein, identified as cellular or tissue transglutaminase II (TGase II). Transglutaminases are enzymes that catalyze the crosslinking of proteins by epsilon-gamma glutamyl lysine isopeptide bonds. While the primary structure of transglutaminases is not conserved, they all have the same amino acid sequence at their active sites and their activity is calcium-dependent. The protein encoded by this gene acts as a monomer, is induced by retinoic acid, and appears to be involved in apoptosis. Finally, the encoded protein is the autoantigen implicated in celiac disease. The identification of transglutaminase as the main antigen of endomysium antibodies allows a new diagnostic approach to celiac disease (CD), a genetic, immunologically mediated small bowel enteropathy that causes malabsorption. TGase II is implicated in programmed cell death, signal transduction, drug-resistance, cell growth, endocytosis, insulin secretion, cell adhesion, cataract formation, and wound healing.

UniProt: P21980

Pathways: Tube Formation, Thromboxane A2 Receptor Signaling