CD28 antibody

Catalog No. ABIN3025653

The Mouse Monoclonal anti-CD28 antibody is suitable to detect CD28 in samples from Human and Mouse. It has been validated for FACS, IF and Func.

Quick Overview for CD28 antibody (ABIN3025653)

Target: CD28

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD28 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Functional Studies (Func)

Grade: Carrier-free

Clone: 204-12

Product Details anti-CD28 Antibody

Purpose: CD28 Antibody (azide and preservative free)

Purification: Protein G affinity chromatography

Immunogen: Stimulated human leukocytes were used as the immunogen for the CD28 antibody.

Isotype: IgG2a, kappa

Application Details

Application Notes: Optimal dilution of the CD28 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the CD28 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for CD28

Target: CD28

Alternative Name: CD28

Background: Recognizes a glycoprotein of 44-88 kDa, which is identified as CD28. It is the critical T-cell co-stimulatory receptor which provides to the cell the important second activation signal by binding CD80 and CD86 that are expressed by antigen presenting cells. Besides its co-stimulation role, CD28 functions in preventing T-cells from anergic hyporesponsive state or from undergoing premature apoptotic cell death. CD28 is also expressed on human fetal NK cells and some NK cell lines, whereas on murine NK cells the CD28 expression is much broader.

UniProt: P10747

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response