Insulin antibody

Catalog No. ABIN3025691

This Mouse Monoclonal antibody specifically detects Insulin in IF and IHC (p). It exhibits reactivity toward Human, Pig, Mouse, Cow and Rabbit.

Quick Overview for Insulin antibody (ABIN3025691)

Target: Insulin (INS)

Reactivity: Human, Pig, Mouse, Cow, Rabbit

Host: Mouse

Clonality: Monoclonal

Conjugate: This Insulin antibody is un-conjugated

Application: Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: IRDN-805

Product Details anti-Insulin Antibody

No Cross-Reactivity: Rat (Rattus)

Purification: Protein G affinity chromatography

Immunogen: Recombinant INS protein was used as the immunogen for the Insulin antibody.

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal dilution of the Insulin antibody should be determined by the researcher.

1. No special pretreatment is required for staining of formalin/paraffin tissues.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format : incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the Insulin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for Insulin

Target: Insulin (INS)

Alternative Name: Insulin

Background: Recognizes a polypeptide which is identified as insulin, a 51-amino acid polypeptide composed of A and B chains connected through the C-peptide. Proinsulin, which has very little biological activity, is cleaved by proteases within its cell of origin into the insulin molecule and the C-terminal basic residue. Insulin enhances membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage, formation of triglycerides, and synthesis of proteins and nucleic acids. Deficiency of insulin results in diabetes mellitus. The main storage site for insulin is the pancreatic islets. Antibodies to insulin are important as beta-cell and insulinoma marker.

Pathways: NF-kappaB Signaling, RTK Signaling, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Hormone Activity, Carbohydrate Homeostasis, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Autophagy, Negative Regulation of intrinsic apoptotic Signaling, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation