Nucleolin antibody (AA 364-5)

Catalog No. ABIN3025717

The Mouse Monoclonal anti-Nucleolin antibody (Clone 364-5-NCL-902) (ABIN3025717) specifically detects Nucleolin in WB, IF, FACS and IHC (p). The antibody is reactive with Human samples.

Quick Overview for Nucleolin antibody (AA 364-5) (ABIN3025717)

Target: Nucleolin (NCL)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Nucleolin antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Grade: Carrier-free

Clone: 364-5-NCL-902

Product Details anti-Nucleolin Antibody

Binding Specificity: AA 364-5

Purpose: Nucleolin Antibody Cocktail (azide and preservative free)

No Cross-Reactivity: Mouse (Murine), Rat (Rattus), Cow (Bovine)

Purification: Protein G affinity chromatography

Immunogen: SUDHL1 cell nuclear lysate (364-5) and recombinant human protein (NCL/902) were used as the immunogen for the Nucleolin antibody cocktail.

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal dilution of the Nucleolin antibody cocktail should be determined by the researcher.

1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the Nucleolin antibody cocktail at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for Nucleolin

Target: Nucleolin (NCL)

Alternative Name: Nucleolin

Background: Recognizes a protein of ~76 kDa, which is identified as Nucleolin (NCL). It is the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. This mAb can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.

UniProt: P19338

Pathways: Ribonucleoprotein Complex Subunit Organization