Metabotropic Glutamate Receptor 5 antibody (Extracellular Domain, N-Term)

Catalog No. ABIN302583

This anti-Metabotropic Glutamate Receptor 5 antibody is a Rabbit Polyclonal antibody detecting Metabotropic Glutamate Receptor 5 in IHC (p). Suitable for Rat, Human and Mouse.

Quick Overview for Metabotropic Glutamate Receptor 5 antibody (Extracellular Domain, N-Term) (ABIN302583)

Target: Metabotropic Glutamate Receptor 5 (GRM5) (Glutamate Receptor, Metabotropic 5 (GRM5))

Reactivity: Rat, Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Metabotropic Glutamate Receptor 5 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Metabotropic Glutamate Receptor 5 Antibody

Binding Specificity: Extracellular Domain, N-Term

Specificity: This antibody detects Metabotropic Glutamate Receptor 5 (GRM5) at N-terminal extracellular domain.

Purification: Immunoaffinity chromatography

Immunogen: GRM5 antibody was raised against synthetic 17 amino acid peptide from N-terminal extracellular domain of Human GRM5 / MGLUR5.

Application Details

Application Notes: Immunohistochemistry on paraffin sections (5.6 μg/mL). Antigen retrieval: Steam slides in 0.01 M sodium citrate buffer, pH 6.0, at 99-100C for 20 min. Remove from heat and let stand at room temperature in buffer for 20 min. Rinse in 1xTBS with Tween (TBST) for 1 min. at room temperature.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline containing 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C to -70 °C for longer.

Target Details for Metabotropic Glutamate Receptor 5

Target: Metabotropic Glutamate Receptor 5 (GRM5) (Glutamate Receptor, Metabotropic 5 (GRM5))

Alternative Name: mGluR5 / GRM5

Background: Glutamate receptors constitute the principal excitatory neurotransmitter receptors in brain. Two classes of glutamate receptors exist: Ionotropic receptors, and metabotropic receptors (mGluRs). Ionotropic glutamate receptors are oligomeric complexes of various subunits (GluR1 7, NMDA1 3, KAI 2) which comprise ligand gated calcium channels. Metabotropic glutamate receptors are G protein coupled receptors that contain 7 membrane spanning domains and a large extracellular N terminal region. When activated, they can activate phopholipases or adenylylcyclase, depending on the neuron. The group I receptors mGluR1 and mGluR5 activate phospholipase C. By screening a human brain cDNA library with a rat mGluR1 alpha cDNA under low stringency conditions, Minakami et al. (1993) and Minakami et al. (1994) isolated human cDNAs encoding mGluR5. Minakami et al. (1993) identified a longer isoform of mGluR5 (mGluR5B).Synonyms: GPRC1E, MGLUR5, Metabotropic glutamate receptor 5

Gene ID: 2915

NCBI Accession: NP_000833

UniProt: P41594

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling, Regulation of long-term Neuronal Synaptic Plasticity