Cytokeratin 19 antibody

Catalog No. ABIN3025844

This Mouse Monoclonal antibody specifically detects Cytokeratin 19 in WB, IF and IHC (p). It exhibits reactivity toward Human and Rat.

Quick Overview for Cytokeratin 19 antibody (ABIN3025844)

Target: Cytokeratin 19 (KRT19) (Keratin 19 (KRT19))

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cytokeratin 19 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Grade: Carrier-free

Clone: KRT19-800

Product Details anti-Cytokeratin 19 Antibody

Purpose: CK19 Antibody / Cytokeratin 19 (azide and preservative free)

Purification: Protein G affinity chromatography

Immunogen: Recombinant human Cytokeratin 19 protein was used as the immunogen for the CK19 antibody.

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal dilution of the CK19 antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the CK19 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for Cytokeratin 19

Target: Cytokeratin 19 (KRT19) (Keratin 19 (KRT19))

Alternative Name: CK19

Background: Recognizes a protein of 40 kDa, identified as cytokeratin-19 (CK19), which is expressed in sweat gland, mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, and ectocervical epithelium. Anti-CK19 reacts with a wide variety of epithelial malignancies including adenocarcinomas of the colon, stomach, pancreas, biliary tract, liver, and breast. Perhaps the most useful application is the identification of thyroid carcinoma of the papillary type, although 50 % -60 % of follicular carcinomas are also labeled. Anti-CK19 is a useful marker for detection of tumor cells in lymph nodes, peripheral blood, bone marrow and breast cancer.

UniProt: P08727