S100A8/A9 Complex (Calprotectin) antibody

Catalog No. ABIN3026084

This Mouse Monoclonal antibody specifically detects S100A8/A9 Complex (Calprotectin) in IHC (p). It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for S100A8/A9 Complex (Calprotectin) antibody (ABIN3026084)

Target: S100A8/A9 Complex (Calprotectin) (S100A8/A9) (Calprotectin (S100A8/A9))

Reactivity: Human, Rat, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This S100A8/A9 Complex (Calprotectin) antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Grade: Carrier-free

Clone: MAC387

Product Details anti-S100A8/A9 Complex (Calprotectin) Antibody

Purpose: MRP14 Antibody / S100A9 (azide and preservative free)

Purification: Protein G affinity chromatography

Immunogen: An affinity purified monocyte membrane preparation was used as the immunogen for the MRP14 antibody.

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal dilution of the MRP14 antibody should be determined by the researcher.

1. Staining of formalin/paraffin tissues requires boiling tissue sections in 10 mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the MRP14 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for S100A8/A9 Complex (Calprotectin)

Target: S100A8/A9 Complex (Calprotectin) (S100A8/A9) (Calprotectin (S100A8/A9))

Alternative Name: MRP8 + MPR14

Background: Recognizes the L1 or Calprotectin molecule, an intra-cytoplasmic antigen comprising of a 12 kDa alpha chain and a 14 kDa beta chain expressed by granulocytes, monocytes and by tissue macrophages. Macrophages usually arise from hematopoietic stem cells in the bone marrow. Under migration into tissues, the monocytes undergo further differentiation to become multifunctional tissue macrophages. They are classified into normal and inflammatory macrophages. Normal macrophages include macrophages in connective tissue (histiocytes), liver (Kupffer's cells), lung (alveolar macrophages), lymph nodes (free and fixed macrophages), spleen (free and fixed macrophages), bone marrow (fixed macrophages), serous fluids (pleural and peritoneal macrophages), skin (histiocytes, Langerhans's cell) and in other tissues. Inflammatory macrophages are present in various exudates. Macrophages are part of the innate immune system, recognizing, engulfing and destroying many potential pathogens including bacteria, pathogenic protozoa, fungi and helminthes. This MAb reacts with neutrophils, monocytes, macrophages, and squamous mucosal epithelia and has been shown as an important marker for identifying macrophages in tissue sections.

UniProt: P06702

Pathways: S100 Proteins