CDK1 antibody

Catalog No. ABIN3026474

This Mouse Monoclonal antibody specifically detects CDK1 in WB and IHC (p). It exhibits reactivity toward Human.

Quick Overview for CDK1 antibody (ABIN3026474)

Target: CDK1 (Cyclin-Dependent Kinase 1 (CDK1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CDK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Grade: Carrier-free

Clone: POH-1

Product Details anti-CDK1 Antibody

Purpose: CDK1 Antibody / CDC2 (azide and preservative free)

No Cross-Reactivity: Mouse (Murine), Rat (Rattus), Xenopus laevis, Fruit Fly (Drosophila melanogaster), Schizosaccharomyces pombe, Yeast (Saccharomyces cerevisiae)

Characteristics: This antibody recognizes a 34 kDa protein, which is identified as cyclin dependent kinase 1 (Cdk1) or p34cdc2 protein kinase. CDK1 plays a crucial role during cell division and is most active during mitosis. It is predominantly localized in the nucleus. It is a serine/threonine kinase, which is activated by cyclin, presumably by de-phosphorylation of tyrosine residues. Activated CDK1 performs specific functions during mitosis, including nuclear envelope breakdown and chromosome condensation.

Purification: Protein G purified antibody

Immunogen: Purified recombinant human Cdk1 / p34cdc2 was used as the immunogen for this CDK1 antibody.

Isotype: IgG2a, kappa

Application Details

Application Notes: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CDK1 antibody to be titered up or down for optimal performance.

1. Staining of FFPE tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the CDK1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for CDK1

Target: CDK1 (Cyclin-Dependent Kinase 1 (CDK1))

Alternative Name: CDK1

Background: This antibody recognizes a 34 kDa protein, which is identified as cyclin dependent kinase 1 (Cdk1) or p34cdc2 protein kinase. CDK1 plays a crucial role during cell division and is most active during mitosis. It is predominantly localized in the nucleus. It is a serine/threonine kinase, which is activated by cyclin, presumably by de-phosphorylation of tyrosine residues. Activated CDK1 performs specific functions during mitosis, including nuclear envelope breakdown and chromosome condensation.

UniProt: P06493

Pathways: Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Toll-Like Receptors Cascades, Synthesis of DNA