CDK1 antibody

Catalog No. ABIN3026474

This Mouse Monoclonal antibody specifically detects CDK1 in IF, IHC (p) and FACS. It exhibits reactivity toward Human, Monkey, Cow and Mink.

Quick Overview for CDK1 antibody (ABIN3026474)

Target: CDK1 (Cyclin-Dependent Kinase 1 (CDK1))

Reactivity: Human, Monkey, Cow, Mink

Host: Mouse

Clonality: Monoclonal

Conjugate: This CDK1 antibody is un-conjugated

Application: Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

Clone: POH-1

Product Details anti-CDK1 Antibody

No Cross-Reactivity: Mouse (Murine), Rat (Rattus), Xenopus laevis, Fruit Fly (Drosophila melanogaster), Schizosaccharomyces pombe, Yeast (Saccharomyces cerevisiae)

Characteristics: This antibody recognizes a 34 kDa protein, which is identified as cyclin dependent kinase 1 (Cdk1) or p34cdc2 protein kinase. CDK1 plays a crucial role during cell division and is most active during mitosis. It is predominantly localized in the nucleus. It is a serine/threonine kinase, which is activated by cyclin, presumably by de-phosphorylation of tyrosine residues. Activated CDK1 performs specific functions during mitosis, including nuclear envelope breakdown and chromosome condensation.

Purification: Protein G purified antibody

Immunogen: Purified recombinant human Cdk1 / p34cdc2 was used as the immunogen for this Cdk1 antibody.

Isotype: IgG2a kappa

Application Details

Application Notes: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Cdk1 antibody to be titered up or down for optimal performance.

1. Staining of FFPE tissues requires boiling tissue sections in 10  mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min.\. FACS: 0.5-1 μg/million cells,IF: 1-2 μg/mL,IHC (FFPE) only: 1-2 μg/mL (1)

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the Cdk1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for CDK1

Target: CDK1 (Cyclin-Dependent Kinase 1 (CDK1))

Alternative Name: Cdk1

Background: This antibody recognizes a 34  kDa protein, which is identified as cyclin dependent kinase 1 (Cdk1) or p34cdc2 protein kinase. CDK1 plays a crucial role during cell division and is most active during mitosis. It is predominantly localized in the nucleus. It is a serine/threonine kinase, which is activated by cyclin, presumably by de-phosphorylation of tyrosine residues. Activated CDK1 performs specific functions during mitosis, including nuclear envelope breakdown and chromosome condensation.

Gene ID: 983

Pathways: Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Toll-Like Receptors Cascades, Synthesis of DNA