Ku70 + Ku80 antibody (PE)

Catalog No. ABIN3026514

The Mouse Monoclonal anti-Ku70 + Ku80 antibody has been validated for FACS and IF. It is suitable to detect Ku70 + Ku80 in samples from Human and Primate.

Quick Overview for Ku70 + Ku80 antibody (PE) (ABIN3026514)

Target: Ku70 + Ku80 (KU80) (CG18801 Gene Product From Transcript CG18801-RA (KU80))

Reactivity: Human, Primate

Host: Mouse

Clonality: Monoclonal

Conjugate: This Ku70 + Ku80 antibody is conjugated to PE

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: KU729

Product Details anti-Ku70 + Ku80 Antibody (PE)

No Cross-Reactivity: Mouse (Murine), Rat (Rattus), Chicken

Characteristics: This antibody recognizes a dimer of two proteins of 70 kDa (Ku70) and ~80 kDa (Ku80), identified as two subunits of Ku. Antbody KU729 recognizes a conformational epitope of the Ku70 + Ku80 dimer, which is destroyed during Western blotting. The Ku70 + Ku80 dimer is important for function of a 460 kDa DNA-dependent protein kinase. Ku protein plays a role in cell signaling, proliferation, DNA repair, replication, transcriptional activation, and apoptosis.

Purity: Protein G affinity chromatography

Immunogen: Nuclear extract of human HL-60 cells was used as the immunogen for this Ku70 + Ku80 antibody.

Isotype: IgG1 kappa

Application Details

Application Notes: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Ku70 + Ku80 antibody to be titered up or down for optimal performance.\. Flow Cytometry : 5 μL/test/million cells or 5 μL/test/100 μL of whole blood,Immunofluorescence : 1:50-1:100 for 30 minutes at RT

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: 500 μL at 0.1 mg/mL with 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the Ku70 + Ku80 antibody at 2-8°C. Conjugate is light sensitive, store in the dark.

Target Details for Ku70 + Ku80

Target: Ku70 + Ku80 (KU80) (CG18801 Gene Product From Transcript CG18801-RA (KU80))

Alternative Name: Ku70 + Ku80

Gene ID: 2547